Conditions necessary for inhibition of protein synthesis and production of cytopathic effect in Aedes albopictus cells infected with vesicular stomatitis virus.
Conditions necessary for inhibition of protein synthesis and production of cytopathic effect in Aedes albopictus cells infected with vesicular stomatitis virus.
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在感染水疱性口炎病毒的白纹伊蚊细胞中抑制蛋白质合成和产生细胞病变作用所需的条件。
DOI：
10.1128/mcb.2.1.66-75.1982
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发表时间：
1982
影响因子：
5.3
通讯作者：
Stollar,V
中科院分区：
文献类型：
--
作者：
Gillies,S;Stollar,V
The relationship between the development of cytopathic effect (CPE) and the inhibition of host macromolecular synthesis was examined in a CPE-susceptible cloned line ofAedes albopictuscells after infection with vesicular stomatitis virus. To induce rapid and maximal CPE, two conditions were required: (i) presence of serum in the medium and (ii) incubation at 34°C rather than at 28°C. In the absence of serum, incubation of infected cultures at 34°C resulted in a significant increase in viral protein and RNA synthesis compared with that observed at 28°C. However, when serum was present in the medium, by 6 h after infection protein synthesis (both host and viral) was markedly inhibited when infected cells were maintained at 34°C. RNA synthesis (host and viral) was also inhibited in vesicular stomatitis virus-infected cells maintained at 34°C with serum, but somewhat more slowly than protein synthesis. Examination of polysome patterns indicated that when infected cultures were maintained under conditions which predispose to CPE, more than half of the ribosomes existed as monosomes, suggesting that protein synthesis was being inhibited at the level of initiation. In addition, the phosphorylation of one (or two) polysome-associated proteins was reduced when protein synthesis was inhibited. Our findings indicate a strong correlation between virus-induced CPE in the LT-C7 clone ofA. albopictuscells and the inhibition of protein synthesis. Although the mechanism of the serum effect is not understood, incubation at 34°C probably predisposes to CPE and inhibition of protein synthesis by increasing the amount of viral gene products made.
