Controlled Assembly of Bifunctional Chimeric Protein Cages and Composition Analysis Using Noncovalent Mass Spectrometry
Controlled Assembly of Bifunctional Chimeric Protein Cages and Composition Analysis Using Noncovalent Mass Spectrometry
复制标题
DOI：
10.1021/ja807655t
复制
发表时间：
2008-12-10
影响因子：
15
通讯作者：
Douglas, Trevor
中科院分区：
文献类型：
--
作者：
Kang, Sebyung;Oltrogge, Luke M.;Douglas, Trevor
通过以所需比例重组解离的亚基构建在LiDps内部和外部具有双重功能的嵌合蛋白笼，并通过非共价质谱在分子水平上监测其组成。质谱的二项分布分析表明，解离的亚基随机重组成十二聚体笼。
The chimeric protein cages having dual functionalities inside and outside of LiDps are constructed by reassembling dissociated subunits with desired ratios and their compositions are monitored by noncovalent mass spectrometry at the molecular level. Binomial distribution analysis of mass spectra reveals that dissociated subunits reassemble randomly into a dodecameric cage.