ROLE OF RIBOSOMAL PROTEIN-S1 IN PROTEIN-SYNTHESIS - EFFECTS OF ITS ADDITION TO BACILLUS-STEAROTHERMOPHILUS CELL-FREE SYSTEM
ROLE OF RIBOSOMAL PROTEIN-S1 IN PROTEIN-SYNTHESIS - EFFECTS OF ITS ADDITION TO BACILLUS-STEAROTHERMOPHILUS CELL-FREE SYSTEM
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DOI：
10.1111/j.1432-1033.1975.tb02202.x
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发表时间：
1975-01-01
期刊：
影响因子：
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通讯作者：
ISONO, K
中科院分区：
文献类型：
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作者：
ISONO, S;ISONO, K
分析了来自大肠杆菌和嗜热脂肪芽孢杆菌的f2噬菌体RNA指导的蛋白质体外合成系统的产物。与其他报道相比，发现B.当使用同源来源的高速（100000 xg）上清液（S100）和核糖体洗涤液（粗起始因子）时，嗜热脂肪菌核糖体合成噬菌体外壳蛋白。此外，还观察到B.当S100或核糖体洗涤或两者均弗罗姆.使用大肠杆菌细胞代替相应的同源组分。负责这种刺激的原理被确定为存在于S100和核糖体洗涤中的30-S核糖体蛋白S1。弗罗姆.大肠杆菌与核糖体的摩尔比约为1：1，当加入B时，[14 C]缬氨酸的掺入刺激约为10倍。嗜热脂肪系统这种刺激主要表现在外壳蛋白和复制酶的合成上。
Products of the f2 phage RNA‐directed protein synthesizing systemsin vitro, derived fromEscherichia coliandBacillus stearothermophilus, were analyzed. In contrast to other reports, it was found thatB. stearothermophilusribosomes synthesized phage coat protein when the high‐speed (100000 xg) supernatant (S100) and ribosomal wash (crude initiation factors) of homologous origin were used. Furthermore, a marked stimulation of coat protein synthesis was observed withB. stearothermophilusribosomes when either S100or ribosomal wash or both fromE. colicells were used instead of the respective homologous components. The principle responsible for this stimulation was identified as the 30‐S ribosomal protein S1 present in S100and ribosomal wash. Purified S1 fromE. coliat roughly one‐to‐one molar ratio to ribosomes resulted in about 10‐fold stimulation of the incorporation of [14C]valine when added to theB. stearothermophilussystem. This stimulation was observed mainly in the synthesis of coat protein and replicase.