DISTRIBUTION AND CHEMICAL COMPOSITION OF ULTRACENTRIFUGALLY SEPARATED LIPOPROTEINS IN HUMAN SERUM
DISTRIBUTION AND CHEMICAL COMPOSITION OF ULTRACENTRIFUGALLY SEPARATED LIPOPROTEINS IN HUMAN SERUM
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DOI：
10.1172/jci103182
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发表时间：
1955-01-01
影响因子：
15.9
通讯作者：
BRAGDON, JH
中科院分区：
文献类型：
--
作者：
HAVEL, RJ;EDER, HA;BRAGDON, JH
逐步提高溶剂密度后，通过反复超离心从血清中分离脂蛋白组分。分离出3种组分：密度小于1.019的脂蛋白，密度在1.019和1.063之间的脂蛋白，以及密度大于1.063的脂蛋白。年轻人携带约2/3的血清胆固醇和约一半的磷脂在2d部分。第一部分中的脂质量在年轻男性中大于年轻女性。年轻男性的3d部分中的脂质量低于年轻女性。在高脂蛋白血症中，第一、第二或两者的分数都升高。3d部分受到影响。馏分的化学组成的改变也发生在疾病中。在动物血清中，不同种属之间的分布和组成存在很大差异。总脂质的更大百分比出现在3d部分中。在人的3d部分的亚分级显示2个部分不同，仅在蛋白质含量。在1.21的密度下离心后，没有胆固醇残留在Infranate中，其仍然含有约10至15%的脂质P。
Lipoprotein fractions are separated from serum by repeated ultracentrifugations after progressively raising the solvent density. Routinely 3 fractions were separated: lipoproteins with densities less than 1.019, with densities between 1.019 and 1.063, and with densities greater than 1.063. Young adults carry about 2/3 of the serum cholesterol and about half the phospholipid in the 2d fraction. The quantity of lipid in the 1st fraction is greater in young men than in young women. The quantity of lipid in the 3d fraction is less in young men than in young women. In hyperlipoproteinemia either the 1st, 2d, or both, fractions are elevated. The 3d fraction is variably affected. Alterations in chemical composition of the fractions also occur in disease. In animal sera wide variations in distribution and composition occur among the different species. A greater percent of the total lipid occurs in the 3d fraction. Subfractionation of the 3d fraction in man demonstrates 2 fractions differing only in protein content. After centrifugation at a density of 1.21 no cholesterol remains in the infranate, which still contains about 10 to 15% of the lipid P.