Toxicological evaluation of micafungin ophthalmic solution in rabbit eyes
Toxicological evaluation of micafungin ophthalmic solution in rabbit eyes
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DOI：
10.1089/jop.2005.21.149
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发表时间：
2005-04-01
影响因子：
2.3
通讯作者：
Oshika, T
中科院分区：
文献类型：
--
作者：
Hiraoka, T;Wakabayashi, T;Oshika, T
尚无米卡芬净眼部局部给药的报告。本研究的目的是评价0.1%米卡芬净滴眼液在兔眼局部滴注的安全性。在新西兰白色家兔（n = 6）中，将50 μ L 0.1%米卡芬净溶液局部滴注至一只眼睛，并将50 μ L无菌生理盐水应用于另一只眼睛。两种滴眼液均从上午7点开始每小时给药，持续7天。测量角膜厚度、眼内压、内皮细胞密度和泪液样本的乳酸脱氢酶（LDH）活性。裂隙灯生物显微镜和组织病理学检查的眼睛。局部应用米卡芬净1周未引起眼内压、内皮细胞密度和泪液LDH的任何变化。滴注后米卡芬净组的角膜厚度略小于对照组，但显著小于对照组（P = 0.0156，配对t检验），但这种差异在最后一次滴注后24小时内消失。生物显微镜检查和组织病理学显示米卡芬净应用对角膜无显著毒性影响。局部滴注米卡芬净溶液对角膜无明显毒性。这些结果保证了未来对米卡芬净滴眼液抗角膜真菌感染有效性的研究。
There have been no reports of the topical application of micafungin to the eye. The aim of this study was to evaluate the safety of topical instillation of 0.1% micafungin ophthalmic solution in rabbit eyes. In New Zealand white rabbits (n = 6), 50 mu L of 0.1% micafungin solution was topically instilled to 1 eye, and 50 mu L of sterile saline was applied to the other eye. Both eyedrops were administered hourly from 7 A.M. for 7 days. Measurements were conducted on corneal thickness, intraocular pressure, endothelial cell density, and lactate dehydrogenase (LDH) activity of tear samples. The eyes were examined slit-lamp biomicroscopically and histopathologically. Topical micafungin application for 1 week did not induce any changes in intraocular pressure, endothelial cell density, and tear LDH. Corneal thickness after instillation was slightly, but significantly, smaller in the micafungin group than in the control group (P = 0.0156, paired t test), but this difference disappeared within 24 hours after the final instillation. Biomicroscopy and histopathology revealed no significant toxic influence of micafungin application on the cornea. Topical instillation of micafungin solution had no apparent toxicity to the cornea. These results warrant future studies on the efficacy of micafungin ophthalmic solution against corneal fungal infection.