Site-specific regulation of gene expression by estrogen in the hypothalamus of adult female rats
Site-specific regulation of gene expression by estrogen in the hypothalamus of adult female rats
复制标题
DOI：
10.1016/j.neulet.2008.02.054
复制
发表时间：
2008-05
影响因子：
2.5
通讯作者：
Qingfu Xu;T. Hamada;R. Kiyama;Y. Sakuma;Y. Wada‐Kiyama
中科院分区：
文献类型：
--
作者：
Qingfu Xu;T. Hamada;R. Kiyama;Y. Sakuma;Y. Wada‐Kiyama
雌激素在成年雌性大鼠视前区（POA）和下丘脑腹内侧核（VMH）的神经内分泌系统中起重要作用。在卵巢切除后，用/不用雌激素处理7周龄大鼠，并检测四种雌激素应答性神经系统相关基因，编码α 4神经元烟碱乙酰胆碱受体（Chrna 4）、GABA A受体δ（Gabrd）、5-羟色胺受体6（Htr 6）和GABA转运蛋白2（Slc 6a 13），通过实时荧光定量RT-PCR和Western blot分析，研究了雌激素对前半部分含有POA和后半部分含有VMH之间的差异调节。我们进一步研究了Bax，Bcl 2和Prkce，前两个基因参与Chrna 4的基因表达网络，后者参与Gabrd的基因表达网络。雌激素对Bax和Bcl 2的调节在前、后两部分存在差异。结果表明，这些神经系统相关基因的差异调节雌激素之间的前部和后部的下丘脑，并建议的基因表达网络的作用，为各自的基因在成年雌性大鼠的神经内分泌系统。
Estrogen plays critical roles in the neuroendocrine system of adult female rats through separate actions, respectively, in the preoptic area (POA) and the ventromedial nucleus of the hypothalamus (VMH). Seven-week-old rats were treated with/without estrogen after they were ovariectomized, and four estrogen-responsive, neuronal system-related genes, encoding alpha4 neuronal nicotinic acetylcholine receptor (Chrna4), GABAAreceptor delta (Gabrd), serotonin receptor 6 (Htr6), and GABA transporter 2 (Slc6a13), were investigated by real-time RT-PCR and Western blot analyses to examine their differential regulation by estrogen between the anterior part containing POA and the posterior part containing VMH. We further examined Bax, Bcl2, and Prkce, the former two genes to be involved in the gene expression network of Chrna4 and the latter gene, that of Gabrd. The regulation of Bax and Bcl2 by estrogen differed between the anterior and posterior parts. The results demonstrated differential regulation of these neuronal system-related genes by estrogen between the anterior and posterior parts of the hypothalamus and suggested the roles of gene expression networks for the respective genes in the neuroendocrine system of adult female rats.