An inexpensive, fast and sensitive quantitative lateral flow magneto-immunoassay for total prostate specific antigen.
An inexpensive, fast and sensitive quantitative lateral flow magneto-immunoassay for total prostate specific antigen.
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DOI：
10.3390/bios4030204
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发表时间：
2014-09
期刊：
影响因子：
--
通讯作者：
Luxton R
中科院分区：
文献类型：
--
作者：
Barnett JM;Wraith P;Kiely J;Persad R;Hurley K;Hawkins P;Luxton R
我们描述了一个设备的检测特性的谐振线圈磁力计（RCM），以量化顺磁性颗粒（PMP）的免疫层析（侧流）测定。使用PMP开发侧流测定法用于测量血清样品中的总前列腺特异性抗原（PSA）。使用RCM时，总PSA的检测限为0.8 ng/mL，且处于临床显著浓度。在一项初步研究中，从血清样品分析中获得的数据与市售免疫测定法的比较显示出良好的一致性。总PSA的定量磁免疫测定的侧流格式的发展表明RCM与许多免疫测定格式一起操作的潜力。RCM有可能进行修改，以量化这种格式的多种分析物。这项研究显示出一种廉价的设备，能够定量多种分析物在床旁的护理使用磁免疫测定在侧流格式的发展前景。
We describe the detection characteristics of a device the Resonant Coil Magnetometer (RCM) to quantify paramagnetic particles (PMPs) in immunochromatographic (lateral flow) assays. Lateral flow assays were developed using PMPs for the measurement of total prostate specific antigen (PSA) in serum samples. A detection limit of 0.8 ng/mL was achieved for total PSA using the RCM and is at clinically significant concentrations. Comparison of data obtained in a pilot study from the analysis of serum samples with commercially available immunoassays shows good agreement. The development of a quantitative magneto-immunoassay in lateral flow format for total PSA suggests the potential of the RCM to operate with many immunoassay formats. The RCM has the potential to be modified to quantify multiple analytes in this format. This research shows promise for the development of an inexpensive device capable of quantifying multiple analytes at the point-of-care using a magneto-immunoassay in lateral flow format.