DEVELOPMENT OF BLOOD-COAGULATION - A FETAL LAMB MODEL
DEVELOPMENT OF BLOOD-COAGULATION - A FETAL LAMB MODEL
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DOI：
10.1203/00006450-198107000-00014
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发表时间：
1981-01-01
影响因子：
3.6
通讯作者：
CLARKE, WR
中科院分区：
文献类型：
--
作者：
KISKER, CT;ROBILLARD, JE;CLARKE, WR
To study the normal development of blood coagulation factor activities in a growing fetus while avoiding the effects of labor and delivery, a chronic fetal lamb model was developed in which serial blood samples from 10 fetuses were studied during the 3rd trimester of pregnancy and 24 h after birth. Under operating room conditions with sterile technique, a polyethylene catheter with heparin was found to internal and external surfaces was inserted into the femoral artery of the fetus. The catheter was brought out through a skin pouch to the side of the ewe and enclosed in a zip-lock bag. Blood samples were withdrawn from the catheter 3 times/wk for measurement of coagulation factor activities. Levels of coagulation factor activities at birth in noncatheterized animals were not different from those found in catheterized animals, except for factor IX activity which was 12% higher in the catheterized animals (0.02 < P < 0.05). The patterns of development for each of the coagulation factors were similar in all 10 animals. Fibrinogen, prothrombin and factor VII show a decrease in activity early in the last trimester of pregnancy; other factors [V, VIII, IX, X, XI, XII and XIII] show a gradual increase in activity throughout the last trimester of pregnancy. Factors VIII and IX show a significant increase in activity (23% factor VIII and 12% factor IX) associated with the process of delivery. The levels of coagulation factor activities at birth in the lamb relative to adult sheep normals are similar to those found in humans with the exception of factor XIII. Factor XIII is at normal levels in the newborn lamb and is reported to be at levels .apprx. 50% of the adult level in human infants.
