Lesion Profiling at Primary Isolation in RIII Mice is Insufficient in Distinguishing BSE from Classical Scrapie
Lesion Profiling at Primary Isolation in RIII Mice is Insufficient in Distinguishing BSE from Classical Scrapie
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DOI：
10.1111/j.1750-3639.2009.00273.x
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发表时间：
2010-03-01
期刊：
影响因子：
6.4
通讯作者：
Spiropoulos, John
中科院分区：
文献类型：
--
作者：
Beck, Katy E.;Chaplin, Melanie;Spiropoulos, John
在RIII小鼠中牛海绵状脑病（BSE）的初步分离产生的病变特征被认为是可重现的，并且与经典瘙痒病产生的病变特征不同。该图谱的特征在于在灰质区域1、4和7（背侧髓质、下丘脑和隔核）处的峰，其用于在初级分离时诊断BSE。本研究的目的是调查BSE因子是否可能存在于诊断为经典瘙痒症的绵羊中，使用RIII小鼠的病变特征作为判别方法。1996年至1999年间，从各个农场收集了62例阳性瘙痒症田间病例，并对RIII小鼠进行了生物测定。这些分离株中有55株成功地传播给至少一只小鼠。在产生足够数据以允许病变轮廓分析的31个中，10个显示出在脑区1、4和7处具有峰的一致轮廓。该亚组的所有接种物均来自基因型ARQ/ARQ的绵羊。虽然1-4-7-羊瘙痒症特征在初次分离时表现出与RIII小鼠中BSE的相似性，但基于组织病理学、免疫组织化学和聚类分析，它是可区分的。我们的结论是，应谨慎区分此配置文件从BSE和其他参数应考虑达到最终诊断。
Primary isolation of bovine spongiform encephalopathy (BSE) in RIII mice generates a lesion profile believed to be reproducible and distinct from that produced by classical scrapie. This profile, which is characterized by peaks at gray matter areas 1, 4 and 7 (dorsal medulla, hypothalamus and septal nuclei), is used to diagnose BSE on primary isolation. The aim of this study was to investigate whether the BSE agent could be present in sheep diagnosed with classical scrapie, using lesion profiles in RIII mice as a discriminatory method. Sixty-two positive scrapie field cases were collected from individual farms between 1996 and 1999 and bioassayed in RIII mice. Fifty-five of these isolates transmitted successfully to at least one mouse. Of the 31 that produced adequate data to allow lesion profile analysis, 10 showed a consistent profile with peaks at brain areas 1, 4 and 7. All inocula for this subgroup were derived from sheep of genotype ARQ/ARQ. While the 1-4-7-scrapie profile exhibited similarities to BSE in RIII mice at primary isolation, it was distinguishable based on histopathology, immunohistochemistry and cluster analysis. We conclude that caution should be taken to distinguish this profile from BSE and that additional parameters should be considered to reach a final diagnosis.