Formation of early-light-inducible-protein complexes and status of xanthophyll levels under high light and cold stress in barley (Hordeum vulgare L.)
Formation of early-light-inducible-protein complexes and status of xanthophyll levels under high light and cold stress in barley (Hordeum vulgare L.)
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DOI：
10.1007/s004250050589
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发表时间：
1999-06
期刊：
影响因子：
4.3
通讯作者：
M. Montané;Björn Petzold;K. Kloppstech
中科院分区：
文献类型：
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作者：
M. Montané;Björn Petzold;K. Kloppstech
在我们之前的工作中，我们发现大麦在适应强光和冷联合胁迫的过程中积累了大量的早期光诱导蛋白（ELIPs），这种积累优先发生在叶片的顶端部分(m.h。montan<e:1>等人。中国生物医学工程学报，1997,32(2):391 - 391。在相同条件下，我们研究了适应对大麦叶片类囊体膜蛋白和色素组成的影响，特别是对叶黄素和叶绿素的影响及其在叶片中的分布。观察到，高光通量似乎有利于光系统II （LHC II）的三聚体化，而冷光通量似乎有利于LHC II的单体化。强光、低温或两者共同作用对类囊体膜的蛋白质组成影响不大，但LHC II的蛋白质在25°C强光下比在5°C强光下减少得更大。叶黄素总循环类胡萝卜素含量随细胞发育呈线性增加，叶尖部分含量最高。冷光和强光协同作用，诱导总叶黄素含量增加不到一倍，而叶绿素含量基本保持不变。花黄质与玉米黄质组成的组分在5℃下比在25℃下高4 ~ 5倍。如前所述（montan<e:1>等人）。1997年)，同样的条件导致了15倍的增加积累的elip。因此，无论是总叶黄素还是花黄质+玉米黄质在叶片上的分布都不遵循与ELIP相同的模式。因此，叶黄素的积累不能与叶黄素的积累进行化学计量相关。在存在十烷基麦芽糖的情况下，通过电泳，我们首次证明了位于类囊体膜未堆叠的基质层层区域的> - 100 kDa的高分子质量复合物中含有13.5 kDa的ELIPs。
In our previous work we found considerable accumulation of early light-inducible proteins (ELIPs) in barley during adaptation to combined high light and cold stress, an accumulation which occurred preferentially in the apical part of the leaves (M.-H. Montané et al., 1997, Planta 202: 293–302). Here we studied, under the same conditions, the effect of adaptation on the composition of thylakoid membrane proteins and pigments, particularly xanthophylls and chlorophyll, and their distribution within the barley leaf. It was observed that high light fluxes appeared to favour the trimerization of the light-harvesting complex of photosystem II (LHC II) whereas cold appeared to favour the monomers of LHC II. High light, cold or the combination of both factors had only a small effect on the protein composition of the thylakoid membranes except for the proteins of LHC II which were found to decrease under high light to a greater extent at 25 °C than at 5 °C. The total xanthophyll-cycle carotenoid content increased linearly with cellular development, the highest amount being observed in the apical part of the leaf. Cold and high light acted synergistically to induce less than a doubling in the amount of total xanthophylls, while chlorophyllsaandbremained nearly constant. The fraction consisting of antheraxanthin plus zeaxanthin was up to 4- to 5-fold higher at 5 °C than at 25 °C. As determined previously (Montané et al. 1997), the same conditions caused a 15-fold increase in the accumulation of ELIPs. Consequently, neither the distribution of total xanthophylls nor that of antheraxanthin plus zeaxanthin along the leaf followed the same pattern as ELIP. Thus, the accumulation of xanthophylls cannot be stoichiometrically correlated with that of ELIPs. Using electrophoresis in the presence of decylmaltoside, we could demonstrate for the first time that ELIPs of 13.5 kDa are contained in high-molecular-mass complexes of >100 kDa, which are located in the unstacked stroma lamellar region of the thylakoid membranes.