The rice RAD51C gene is required for the meiosis of both female and male gametocytes and the DNA repair of somatic cells
The rice RAD51C gene is required for the meiosis of both female and male gametocytes and the DNA repair of somatic cells
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DOI：
10.1093/jxb/ers190
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发表时间：
2012-09-01
影响因子：
6.9
通讯作者：
Wang, Shiping
中科院分区：
文献类型：
--
作者：
Kou, Yanjun;Chang, Yuxiao;Wang, Shiping
水稻RecA/RAD 51家族由至少13个成员组成。然而，这些成员中的大多数的功能是未知的。在这里的功能特性的一个成员，这个家庭，RAD 51 C，报告。RAD 51 C基因的敲除（KO）导致水稻的雌性和雄性不育。将RAD 51 C转移到RAD 51 C-KO系恢复了育性。细胞学分析表明，RAD 51 C-KO植株的不育性与大孢子母细胞和花粉母细胞减数分裂早期异常有关。PMC没有正常的粗线期染色体，并有异常的染色体片段。RAD 51 C-KO株系在花药壁细胞有丝分裂方面与野生型株系无明显差异，这与RAD 51 C-KO株系在营养生长发育过程中形态无明显异常的观察结果相一致。然而，RAD 51 C-KO系对不同的DNA损伤剂敏感。这些结果表明，RAD 51 C是必不可少的生殖发育，通过调节减数分裂，以及在体细胞DNA损伤修复。
The RecA/RAD51 family of rice (Oryza sativa) consists of at least 13 members. However, the functions of most of these members are unknown. Here the functional characterization of one member of this family, RAD51C, is reported. Knockout (KO) of RAD51C resulted in both female and male sterility in rice. Transferring RAD51C to the RAD51C-KO line restored fertility. Cytological analyses showed that the sterility of RAD51C-KO plants was associated with abnormal early meiotic processes in both megasporocytes and pollen mother cells (PMCs). PMCs had an absence of normal pachytene chromosomes and had abnormal chromosome fragments. The RAD51C-KO line showed no obvious difference from wild-type plants in mitosis in the anther wall cells, which was consistent with the observation that the RAD51C-KO line did not have obviously abnormal morphology during vegetative development. However, the RAD51C-KO line was sensitive to different DNA-damaging agents. These results suggest that RAD51C is essential for reproductive development by regulating meiosis as well as for DNA damage repair in somatic cells.