Alginate bead fabrication and encapsulation of living cells under centrifugally induced artificial gravity conditions
Alginate bead fabrication and encapsulation of living cells under centrifugally induced artificial gravity conditions
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DOI：
10.1080/02652040801954333
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发表时间：
2008-01-01
影响因子：
3.9
通讯作者：
Ducree, Jens
中科院分区：
文献类型：
--
作者：
Haeberle, Stefan;Naegele, Lars;Ducree, Jens
这项研究提出了一种在聚合物管微喷嘴上直接离心诱导制造小型 Ca2+ 硬化藻酸盐珠的新方法。珠子直径可通过喷嘴几何形状在 180-800 m 之间任意调节，旋转频率在 5-28 Hz 之间。主峰的尺寸分布仅具有 7-16% 的 CV。每秒最多 600 个珠子和通道从微喷嘴通过气隙射向标准实验室管（“Eppi”）中包含的固化剂。多个管子可以安装在“飞斗”转子上，它们在旋转时水平对齐，并在转子静止后立即返回垂直位置。离心诱导的超高人造重力条件（高达 180 克）甚至可以对粘度高达 50 Pa s 的海藻酸盐溶液进行微胶囊化，即类似于水粘度的 50 000 倍！通过这种低成本的微胶囊化技术，HN25和PC12细胞已成功被胶囊化，同时保持活力。
This study presents a novel method for the direct, centrifugally induced fabrication of small, Ca2+-hardened alginate beads at polymer-tube micronozzles. The bead diameter can arbitrarily be adjusted between 180-800 m by the nozzle geometry and spinning frequencies between 5-28 Hz. The size distribution of the main peak features a CV of 7-16%, only. Up to 600 beads per second and channel are issued from the micronozzle through an air gap towards the curing agent contained in a standard lab tube ('Eppi'). Several tubes can be mounted on a 'flying bucket' rotor where they align horizontally under rotation and return to a vertical position as soon as the rotor is at rest. The centrifugally induced, ultra-high artificial gravity conditions (up to 180 g) even allow the micro-encapsulation of alginate solutions displaying viscosities up to 50 Pa s, i.e. similar to 50 000 times the viscosity of water! With this low cost technology for microencapsulation, HN25 and PC12 cells have successfully been encapsulated while maintaining vitality.