The Omega Analysis and the Characterization of Solute Self-Association by Sedimentation Equilibrium
The Omega Analysis and the Characterization of Solute Self-Association by Sedimentation Equilibrium
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沉积平衡的 Omega 分析和溶质自缔合表征
DOI：
10.1007/978-1-4684-6828-1_4
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
P. Wills
中科院分区：
文献类型：
--
作者：
D. Winzor;P. Wills
多年来，分析超离心被认为是大分子的异质性和分子量的主要信息来源，这一作用现在已被凝胶电泳和凝胶色谱技术所取代。分析超离心在蛋白质化学中的主要用途是测定大分子相互作用的平衡常数。传统的方法，以ultracentrance分析无疑影响最初尝试采用ultracentrance的特点溶质自缔合。这些分析基于沉降平衡分布得出的重均分子量的浓度依赖性（亚当斯和威廉姆斯，1964; Roark和Yphantis，1969; Hoagland和Teller，1969）。虽然这种做法仍然盛行（Bucci，1986; Chatelier和Minton，1987; Minton，1990），但也开发了更直接的方法来分析沉降平衡分布，这是总溶质浓度c（r）的实验记录，作为径向距离r的函数。
For many years the analytical ultracentrifuge was considered to be the major source of information on the heterogeneity and molecular weight of macromolecules, a role that has now been taken over largely by gel electrophoretic and gel chromatographic techniques. The main use of analytical ultracentrifugation in protein chemistry is now the determination of equilibrium constants for macromolecular interactions. The traditional approach to ultracentrifuge analysis undoubtedly influenced initial attempts to employ the ultracentrifuge for characterizing solute self-association. Those analyses were based on the concentration-dependence of weight-average molecular weights derived from sedimentation equilibrium distributions (Adams and Williams, 1964; Roark and Yphantis, 1969; Hoagland and Teller, 1969). Although such practices still prevail (Bucci, 1986; Chatelier and Minton, 1987; Minton, 1990), more direct procedures have also been developed for analyzing the sedimentation equilibrium distribution, which is an experimental record of total solute concentration, c(r), as a function of radial distance, r.
影响因子：
3.4
作者：
JOHNSON, ML;CORREIA, JJ;HALVORSON, HR
通讯作者：
HALVORSON, HR
可用于分析自缔合系统的沉降平衡数据的平均数量。
影响因子：
3.8
作者：
Bucci,E
通讯作者：
Bucci,E