A Nanoneedle can be Inserted into a Living Cell without Any Mechanical Stress Inducing Calcium Ion Influx
A Nanoneedle can be Inserted into a Living Cell without Any Mechanical Stress Inducing Calcium Ion Influx
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纳米针可以插入活细胞中，没有任何机械应力诱导钙离子流入
DOI：
10.5796/electrochemistry.76.586
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发表时间：
2008
期刊：
影响因子：
2.5
通讯作者：
J. Miyake
中科院分区：
文献类型：
--
作者：
C. Nakamura;Hideki Kamiishi;Noriyuki Nakamura;J. Miyake
当细胞通过压缩、缩进或拉伸而机械变形时，钙离子流入活细胞。钙离子通过机械敏感通道感知变形信号。钙离子是细胞骨架结构重建的关键触发因素。在显微注射过程中，毛细管对细胞的压痕会导致钙离子的流入，就像典型的AFM针尖一样。纳米针是直径为200 nm的尖锐针状AFM针尖，当其插入细胞中时不会引起任何钙离子的流入。来自纳米针插入的机械应力是可忽略的，并且其侵入性低。
An influx of calcium ions into a living cell occurs when the cell is deformed mechanically by compressing, indenting or stretching. The calcium ions are taken up through mechanosensitive channels sensing deforming signals. The calcium ion is a key trigger for the reformation of cytoskeletal structure. Indentation of a capillary to a cell during microinjection causes influx of calcium ions, as does a typical AFM tip. A nanoneedle, which is a sharpened needle shaped AFM tip of 200 nm in diameter does not cause any influx of calcium ions when it is inserted into a cell. The mechanical stress from the nanoneedle insertion is negligible and its invasiveness is low.