Identification of New World Quails Susceptible to Infection with Avian Leukosis Virus Subgroup J
Identification of New World Quails Susceptible to Infection with Avian Leukosis Virus Subgroup J
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DOI：
10.1128/jvi.02002-16
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发表时间：
2017-02-01
影响因子：
5.4
通讯作者：
Hejnar, Jiri
中科院分区：
文献类型：
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作者：
Plachy, Jiri;Reinisova, Marketa;Hejnar, Jiri
J亚群禽白血病病毒（ALV-J）感染家禽、原鸡和火鸡。该病毒通过由tvj基因座编码的受体进入宿主细胞，并被鉴定为Na+/H+交换器1。绝大多数鸡形种对J亚群禽白血病病毒的抗性可以通过Na+/H+交换器1的第一胞外环中的关键色氨酸38的缺失或取代来解释。由于存在跨物种病毒传播的担忧，我们研究了野生鸡的自然多态性和易感性/抗性，并在四种新世界鹌鹑中发现了色氨酸38的存在。新世界鹌鹑的胚胎成纤维细胞对禽白血病病毒J亚群感染敏感，克隆的Na+/H+交换蛋白1赋予了对其他抗性宿主的易感性。新世界鹌鹑也对新的禽白血病病毒J亚群变种易感，但对A亚群和B亚群具有抗性，并且由于各自受体的明显缺陷，对禽肉瘤/白血病病毒C亚群和D亚群易感性较弱。我们的研究结果表明，J亚群禽白血病病毒可以传播给新世界鹌鹑，并建立一个自然水库的循环病毒与进一步evolution.IMPORTANCE的潜力，因为它在中国和东南亚的肉鸡在2000年的传播，ALV-J仍然是一个重大的地方性挑战，家禽业。虽然病毒在家禽中迅速多样化，但大多数物种对ALV-J的抗性阻止了其在野鸟物种中的溢出和传播。然而，了解病毒的演变及其在野鸟中的潜在宿主范围是重要的。因为抵抗禽逆转录病毒是由于特别是受体的不相容性，我们研究了Na+/H+交换器1，ALV-J的受体。在新世界鹌鹑，我们发现了一个兼容的受体与病毒进入，我们证实了四个新世界鹌鹑物种在体外的亲和性。我们建议进行前瞻性分子流行病学研究，以确定有潜力成为ALV-J水库的物种。
The J subgroup of avian leukosis virus (ALV-J) infects domestic chickens, jungle fowl, and turkeys. This virus enters the host cell through a receptor encoded by the tvj locus and identified as Na+/H+ exchanger 1. The resistance to avian leukosis virus subgroup J in a great majority of galliform species has been explained by deletions or substitutions of the critical tryptophan 38 in the first extracellular loop of Na+/H+ exchanger 1. Because there are concerns of transspecies virus transmission, we studied natural polymorphisms and susceptibility/resistance in wild galliforms and found the presence of tryptophan 38 in four species of New World quails. The embryo fibroblasts of New World quails are susceptible to infection with avian leukosis virus subgroup J, and the cloned Na+/H+ exchanger 1 confers susceptibility on the otherwise resistant host. New World quails are also susceptible to new avian leukosis virus subgroup J variants but resistant to subgroups A and B and weakly susceptible to subgroups C and D of avian sarcoma/leukosis virus due to obvious defects of the respective receptors. Our results suggest that the avian leukosis virus subgroup J could be transmitted to New World quails and establish a natural reservoir of circulating virus with a potential for further evolution.IMPORTANCE Since its spread in broiler chickens in China and Southeast Asia in 2000, ALV-J remains a major enzootic challenge for the poultry industry. Although the virus diversifies rapidly in the poultry, its spillover and circulation in wild bird species has been prevented by the resistance of most species to ALV-J. It is, nevertheless, important to understand the evolution of the virus and its potential host range in wild birds. Because resistance to avian retroviruses is due particularly to receptor incompatibility, we studied Na+/H+ exchanger 1, the receptor for ALV-J. In New World quails, we found a receptor compatible with virus entry, and we confirmed the susceptibilities of four New World quail species in vitro. We propose that a prospective molecular epidemiology study be conducted to identify species with the potential to become reservoirs for ALV-J.