Ethylene increases accumulation of compatible solutes and decreases oxidative stress to improve plant tolerance to water stress in Arabidopsis
Ethylene increases accumulation of compatible solutes and decreases oxidative stress to improve plant tolerance to water stress in Arabidopsis
复制标题
乙烯增加了相容性溶质的积累并减少了氧化应激，从而提高了拟南芥植物对水分胁迫的耐受性
DOI：
10.1007/s12374-014-0302-z
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发表时间：
2015-06-01
影响因子：
2.9
通讯作者：
Tang, Zhonghua
中科院分区：
文献类型：
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作者：
Cui, Mengying;Lin, Yingchao;Tang, Zhonghua
乙烯（ET）是植物响应外界刺激的重要信号转导分子。在水分亏缺条件下，植物激素对渗透和氧化还原状态的精细调节对植物抗旱性至关重要。在此，ET不敏感突变体（ein 2 -5和ein 3 -1）在水分胁迫下，聚乙二醇6000（PEG-6000）刺激的生理和细胞反应进行了分析，并与野生型植物（Col-0）作为对照进行了比较。Col-0中相容性溶质的积累高于ein 2 -5和ein 3 -1。在Col-0植物中，水分胁迫也增加了P5 CS 1的转录，P5 CS 1编码脯氨酸生物合成中的关键限速酶。这些结果表明，ET信号参与了增加可溶性糖和脯氨酸积累的上调，以适应渗透胁迫。此外，ET缺陷型突变体中的氧化应激高于Col-0野生型植物。同时，活性氧（ROS）清除酶超氧化物歧化酶（SOD）和过氧化物酶（POD）的活性增加，观察在ET不敏感突变体，表明ET缺陷植物在干旱条件下加重氧化胁迫。总之，ET信号参与调节植物在干旱条件下的氧化胁迫。
Ethylene (ET) plays an important role as signal transducer in plants in response to environmental stimuli. Under water deficient conditions, fine adjustment of osmosis and redox states through phytohormones is vital for plant resistance to drought. Here, physiological and cellular responses of ET insensitive mutants (ein2-5 and ein3-1) were analyzed under water deficiency stimulated by polyethylene glycol 6000 (PEG-6000), and compared with wild type plants (Col-0) as controls. The accumulation of compatible solutes was higher in Col-0 than in ein2-5 and ein3-1. In Col-0 plants, water stress also increased transcription of P5CS1, which encoded a key rate-limiting enzyme in proline biosynthesis. These results suggested that ET signaling is involved in increasing the up-regulation of soluble sugar and proline accumulation to adjust to osmotic stress. In addition, oxidative stress was higher in ET defective mutants than in Col-0 wild-type plants. Meanwhile, increased activities of the reactive oxygen species (ROS)-scavenging enzymes superoxide dismutase (SOD) and peroxidase (POD) were observed in ET insensitive mutants, indicating aggravated oxidative stress in ET-defective plants under drought. In conclusion, ET signaling is involved in modulating plant oxidative stress under drought conditions.