Chitosan hydrogels enriched with polyphenols: Antibacterial activity, cell adhesion and growth and mineralization
Chitosan hydrogels enriched with polyphenols: Antibacterial activity, cell adhesion and growth and mineralization
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DOI：
10.1016/j.carbpol.2015.04.043
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发表时间：
2015-09-20
影响因子：
11.2
通讯作者：
Bacakova, Lucie
中科院分区：
文献类型：
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作者：
Liskova, Jana;Douglas, Timothy E. L.;Bacakova, Lucie
用于骨再生的可注射水凝胶由壳聚糖、β-甘油磷酸钠 (Na beta-GP) 和碱性磷酸酶 (ALP) 组成，富含多酚间苯三酚 (PG) 和没食子酸 (GA)，并在与骨再生应用相关的特性方面进行物理化学和生物学表征，即凝胶动力学、矿化性、抗氧化特性、抗菌活性、细胞相容性以及支持人体粘附和生长的能力成骨细胞样 MG63 细胞。富含 PG 和 GA 对凝胶动力学和矿化性没有负面影响。 PG 和 GA 都增强了未矿化水凝胶的抗氧化活性。矿化降低了含有 GA 的水凝胶的抗氧化活性。含有 GA、PG 且不含多酚的水凝胶在孵育 1 小时、3 小时和 6 小时后降低了大肠杆菌的集落形成能力，并减缓了液体培养中大肠杆菌的生长 150 分钟。含有 GA 的水凝胶具有细胞毒性，并且比不含多酚的水凝胶更难支持细胞生长。 PG对细胞粘附和生长没有负面影响。 (C) 2015 Elsevier Ltd. 保留所有权利。
Injectable hydrogels for bone regeneration consisting of chitosan, sodium beta-glycerophosphate (Na beta-GP) and alkaline phosphatase (ALP) were enriched with the polyphenols phloroglucinol (PG) and gallic acid (GA) and characterized physicochemically and biologically with respect to properties relevant for applications in bone regeneration, namely gelation kinetics, mineralizability, antioxidant properties, antibacterial activity, cytocompatibility and ability to support adhesion and growth of human osteoblast-like MG63 cells. Enrichment with PG and GA had no negative effect on gelation kinetics and mineralizability. PG and GA both enhanced antioxidant activity of unmineralized hydrogels. Mineralization reduced antioxidant activity of hydrogels containing GA. Hydrogels containing GA, PG and without polyphenols reduced colony forming ability of Escherichia coli after 1 h, 3 h and 6 h incubation and slowed E. coli growth in liquid culture for 150 min. Hydrogels containing GA were cytotoxic and supported cell growth more poorly than polyphenol-free hydrogels. PG had no negative effect on cell adhesion and growth. (C) 2015 Elsevier Ltd. All rights reserved.