Double patch clamp reveals that transient fusion (kiss-and-run) is a major mechanism of secretion in calf adrenal chromaffin cells: High calcium shifts the mechanism from kiss-and-run to complete fusion
Double patch clamp reveals that transient fusion (kiss-and-run) is a major mechanism of secretion in calf adrenal chromaffin cells: High calcium shifts the mechanism from kiss-and-run to complete fusion
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DOI：
10.1523/jneurosci.5275-05.2006
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发表时间：
2006-03-15
影响因子：
5.3
通讯作者：
Artalejo, CR
中科院分区：
文献类型：
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作者：
Elhamdani, A;Azizi, F;Artalejo, CR
短暂融合（“吻后跑”）被认为是中枢神经元和神经内分泌细胞释放递质的一种方式，但与完全融合相比，这种机制的普遍性仍然存在疑问。使用一种新的双膜片钳方法（整个细胞/细胞连接），允许记录单一电容事件，同时刺激在各种条件下，包括动作电位，我们发现瞬时融合是小牛肾上腺染色质细胞的主要分泌模式（> 90%）。将细胞内Ca2+浓度（[Ca](i)）从10 μ M提高到200 μ M会增加完全融合事件的发生率，但代价是短暂融合。阻断快速内吞作用通常会终止短暂的融合事件，也会促进完全融合事件。因此，[Ca](i)控制着瞬时融合和完全融合之间的转变，每一种融合都与不同的内吞作用模式相耦合。
Transient fusion ("kiss-and-run") is accepted as a mode of transmitter release both in central neurons and neuroendocrine cells, but the prevalence of this mechanism compared with full fusion is still in doubt. Using a novel double patch-clamp method (whole cell/cell attached), permitting the recording of unitary capacitance events while stimulating under a variety of conditions including action potentials, we show that transient fusion is the predominant ( > 90%) mode of secretion in calf adrenal chromaffin cells. Raising intracellular Ca2+ concentration ([Ca](i)) from 10 to 200 mu M increases the incidence of full fusion events at the expense of transient fusion. Blocking rapid endocytosis that normally terminates transient fusion events also promotes full fusion events. Thus, [Ca](i) controls the transition between transient and full fusion, each of which is coupled to different modes of endocytosis.