Similar cefoxitin-resistance plasmids circulating in Escherichia coli from human and animal sources
Similar cefoxitin-resistance plasmids circulating in Escherichia coli from human and animal sources
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DOI：
10.1016/j.vetmic.2008.08.018
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发表时间：
2009-03-02
影响因子：
3.3
通讯作者：
Read, R. R.
中科院分区：
文献类型：
--
作者：
Mulvey, M. R.;Susky, E.;Read, R. R.
这项研究的目的是确定在进入饲养场的牛中发现的对头孢西丁耐药的大肠杆菌的分子流行病学，并确定是否与E Call在加拿大医院引起人类感染有任何相似之处。从加拿大阿尔伯塔省南部四个饲养场总共2483头牛中分离出51株大肠杆菌。脉冲场凝胶电泳法的DNA指纹图谱显示了32种独特的模式，观察到两个主要的聚类群，包括聚集群A(11株)和聚集性B(7株)。聚合酶链式反应和序列分析显示，38株(74.5%)携带BLA(CMY-2)，而其余的发现含有与头孢西丁耐药相关的染色体AmpC基因启动子区域的突变。临床分离株对奈利地酸、环丙沙星或阿米卡星均无耐药性。将含质粒的BLA(CMY-2)转入E.所有携带BLA(CMY-2)的质粒都含有A/C复制子，还含有其他抗性基因。用BgIII进行的质粒指纹图谱显示了17种独特的模式，除一种模式外，其余所有模式的相似性都在70%以内。将质粒指纹与在相似时间段内从加拿大从具有临床意义的人类大肠杆菌中分离的那些进行比较[Mulvey，M.R.，Bryce，E.，Boyd，D.A.，Ofner-Agostini，M.，Land，A.M.，Simor，A.E.，Paton，S.，2005。加拿大医院流行病学委员会和加拿大医院感染监测计划，加拿大卫生部。加拿大医院分离的耐头孢西丁大肠埃希菌的分子特征。抗微生物药。化学其他探员。49,358-365]发现四株含有BLA(CMY-2)A/C复制子类型的菌株，其指纹与本研究中从牛中鉴定的E.Call中鉴定的A/C复制子类型的质粒的指纹相似性大于90%。这些发现突显了食品生产动物中的多药耐药微生物与加拿大医院中的人类感染的潜在联系。这些质粒会对多种抗生素产生耐药性，这可能会限制由这些菌株引起的感染的治疗选择。皇冠版权所有(C)2008由爱思唯尔出版。保留所有权利。
The aim of this study was to determine the molecular epidemiology of cefoxitin-resistance Escherichia coli identified in cattle entering feedlots and determine if there were any similarities to E call causing human infections in Canadian hospitals. A total of 51 E. coli were isolated from a total of 2483 cattle entering four feedlots in southern Alberta, Canada. DNA fingerprinting using pulsed-field gel electrophoresis revealed thirty-two unique patterns with two major clusters observed comprised of Cluster A (11 strains) and Cluster B (7 strains). PCR and sequence analysis revealed 38 isolates (74.5%) harboured bla(CMY-2), whereas the remainder were found to contain mutations in the promoter region of the chromosomal ampC gene, which has been previously associated with cefoxitin resistance. No resistance to nalidixic acid, ciprofloxacin, or amikacin was observed in the clinical isolates. bla(CMY-2) harbouring plasmids were transferred to E. call DH10B. All of the plasmids carrying bla(CMY-2) contained the A/C replicon and also harboured other resistance genes. Plasmid fingerprinting using BgIII revealed 17 unique patterns with all but one clustering within 70% similarity. Comparison of the plasmid fingerprints to those isolated from human clinically significant E. coli in Canada during a similar time period [Mulvey, M.R., Bryce, E., Boyd, D.A., Ofner-Agostini, M., Land, A.M., Simor, A.E, Paton, S., 2005. The Canadian Hospital Epidemiology Committee, and The Canadian Nosocomial Infection Surveillance Program, Health Canada. Molecular characterization of cefoxitin resistant Escherichia coli from Canadian hospitals. Antimicrob. Agents Chemother. 49, 358-365] revealed four strains that harboured bla(CMY-2) A/C replicon type plasmid with fingerprint similarities of greater than 90% to the ones identified in E. call from the cattle in this study.These findings highlight the potential linkage of multidrug resistant organisms in food producing animals and human infections in Canadian hospitals. The plasmids conferred resistance to multiple antibiotics which could limit options for the treatment of infections caused by these strains. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.