MKK1 AND MKK2, WHICH ENCODE SACCHAROMYCES-CEREVISIAE MITOGEN-ACTIVATED PROTEIN KINASE-KINASE HOMOLOGS, FUNCTION IN THE PATHWAY MEDIATED BY PROTEIN-KINASE-C
MKK1 AND MKK2, WHICH ENCODE SACCHAROMYCES-CEREVISIAE MITOGEN-ACTIVATED PROTEIN KINASE-KINASE HOMOLOGS, FUNCTION IN THE PATHWAY MEDIATED BY PROTEIN-KINASE-C
复制标题
DOI：
10.1128/mcb.13.5.3076
复制
发表时间：
1993-05-01
影响因子：
5.3
通讯作者：
OSHIMA, Y
中科院分区：
文献类型：
--
作者：
IRIE, K;TAKASE, M;OSHIMA, Y
酿酒酵母的PKC 1基因编码酵母细胞正常生长和分裂所需的哺乳动物蛋白激酶C的同源物。我们在这里报告分离的酵母MKK 1和MKK 2（促分裂原活化蛋白[MAP]激酶激酶）基因，当过表达时，抑制细胞裂解缺陷的温度敏感的pkc 1突变体。MKK基因编码与S.酿酒酵母，粟酒裂殖酵母的副产物，和脊椎动物MAP激酶-激酶。单独缺失任一MKK基因不会引起任何明显的表型缺陷，但缺失MKK 1和MKK 2导致温度敏感性细胞溶解缺陷，其被渗透稳定剂抑制。这种表型缺陷与BCK 1基因缺失相关的表型缺陷相似，BCK 1基因被认为在PKC 1介导的途径中起作用。BCK 1基因还编码预测的蛋白激酶。MKK 1的过表达抑制了BCK 1缺失引起的生长缺陷，而BCK 1的激活等位基因（BCK 1 -20）并没有抑制mkk 1 mkk 2双破坏的缺陷。此外，MPK 1，它编码的蛋白激酶密切相关的脊椎动物MAP激酶的过表达，抑制缺陷的mkk 1 mkk 2双突变体。这些结果表明，MKK 1和MKK 2的功能在一个信号转导途径，涉及蛋白激酶编码的PKC 1，BCK 1和MPK 1。遗传上位性实验表明，MKK 1和MKK 2的作用位点位于BCK 1和MPK 1之间。
The PKC1 gene of Saccharomyces cerevisiae encodes a homolog of mammalian protein kinase C that is required for normal growth and division of yeast cells. We report here the isolation of the yeast MKK1 and MKK2 (for mitogen-activated protein [MAP] kinase-kinase) genes which, when overexpressed, suppress the cell lysis defect of a temperature-sensitive pkc1 mutant. The MKK genes encode protein kinases most similar to the STE7 product of S. cerevisiae, the byr1 product of Schizosaccharomyces pombe, and vertebrate MAP kinase-kinases. Deletion of either MKK gene alone did not cause any apparent phenotypic defects, but deletion of both MKK1 and MKK2 resulted in a temperature-sensitive cell lysis defect that was suppressed by osmotic stabilizers. This phenotypic defect is similar to that associated with deletion of the BCK1 gene, which is thought to function in the pathway mediated by PKC1. The BCK1 gene also encodes a predicted protein kinase. Overexpression of MKK1 suppressed the growth defect caused by deletion of BCK1, whereas an activated allele of BCK1 (BCK1-20) did not suppress the defect of the mkk1 mkk2 double disruption. Furthermore, overexpression of MPK1, which encodes a protein kinase closely related to vertebrate MAP kinases, suppressed the defect of the mkk1 mkk2 double mutant. These results suggest that MKK1 and MKK2 function in a signal transduction pathway involving the protein kinases encoded by PKC1, BCK1, and MPK1. Genetic epistasis experiments indicated that the site of action for MKK1 and MKK2 is between BCK1 and MPK1.