One-bead-one-compound library of end-capped dipeptides and deconvolution by microflow NMR.
One-bead-one-compound library of end-capped dipeptides and deconvolution by microflow NMR.
复制标题
封端二肽的一珠一化合物库和微流 NMR 解卷积。
DOI：
10.1021/cc0500194
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Lokey,RScott
中科院分区：
文献类型：
--
作者：
Simon,RozalynA;Schuresko,Laura;Dendukuri,Nagamani;Goers,Emily;Murphy,Brent;Lokey,RScott
As part of our program to identify novel small molecules with interesting biological activity, we have designed and synthesized a library of end-capped dipeptides with an emphasis on compound diversity, complexity, and membrane permeability. An ∼1500-member library was synthesized manually on large polystyrene beads using the mix-and-split method. The final compounds were cleaved into 384-well plates to generate individual stock solutions for input into high-throughput biological screens. Individual compounds were decoded using a combination of mass spectrometry and microflow NMR spectroscopy. In principle, this approach to deconvolution obviates the need for complicated binary encoding−decoding strategies for one-bead-one-compound libraries.
