Humoral response suppression observed with CD23 transgenics.
Humoral response suppression observed with CD23 transgenics.
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DOI：
10.4049/jimmunol.163.1.217
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发表时间：
1999-07
影响因子：
4.4
通讯作者：
Margaret E. Payet;Elaine C. Woodward;Daniel H. Conrad
中科院分区：
文献类型：
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作者：
Margaret E. Payet;Elaine C. Woodward;Daniel H. Conrad
CD 23，也称为低亲和力IgE受体（FcepsilonRII），已被假设在IgE调节中起作用。使用MHC I类启动子和IgH增强子产生新的CD 23转基因小鼠，以进一步测试CD 23在IgE下调中起作用的假设。通过FACS对三个建立者细胞系的研究表明，在B和T淋巴细胞上都有不同程度的过表达。未观察到淋巴细胞群的变化。所有三个创始人线表现出强烈的抑制IgE响应DNP-keyhole血蓝蛋白/明矾和日本圆线虫巴西感染相比，在父母或同窝对照。表现出最高水平抑制的创始系也不太容易受到银诱导的全身过敏性休克的影响。总体而言，数据支持增强CD 23水平可用于抑制IgE介导的疾病的概念。该机制涉及IgE合成减少，因为IgE的血清半衰期在转基因中没有改变，并且酶联免疫斑点分析表明通过注射抗IgD刺激产生较低IgE的细胞。转基因也表现出显著降低的IgG 1反应，并表现出较低水平的所有IG同种型，虽然这是更可变的不同的创始人线。
CD23, also known as the low affinity IgE receptor (FcepsilonRII), has been hypothesized to have a role in IgE regulation. A new CD23 transgenic mouse was generated using the MHC class I promoter and IgH enhancer to further test the hypothesis that CD23 plays a role in the down-regulation of IgE. Study of three founder lines by FACS showed overexpression to varying extents on both B and T lymphocytes. No alterations in lymphocyte populations was observed. All three founder lines exhibited strong suppression of IgE in response to DNP-keyhole limpet hemocyanin/alum and Nippostrongylus brasiliensis infection compared with that in parental or littermate controls. The founder line exhibiting the highest level of suppression also was less susceptible to Ag-induced systemic anaphylactic shock. Overall, the data support the concept that enhancing CD23 levels can be used to suppress IgE-mediated disease. The mechanism involves decreased IgE synthesis, because the serum half-life of IgE was not altered in transgenics, and enzyme-linked immunospot analysis demonstrated lower IgE-producing cells stimulated by injection of anti-IgD. Transgenics also exhibited significantly decreased IgG1 responses and exhibited lower levels of all Ig isotypes, although this was more variable in different founder lines.