CAAT/enhancer binding proteins directly modulate transcription from the peroxisome proliferator-activated receptor gamma 2 promoter
CAAT/enhancer binding proteins directly modulate transcription from the peroxisome proliferator-activated receptor gamma 2 promoter
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DOI：
10.1006/bbrc.1997.7627
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发表时间：
1997-11-07
影响因子：
3.1
通讯作者：
Gimble, JM
中科院分区：
文献类型：
--
作者：
Clarke, SL;Robinson, CE;Gimble, JM
CCAAT/增强子结合蛋白（C/EBP）和过氧化物酶体增殖物激活受体（PPAR）共同调节脂肪生成。目前的工作使用共转染研究来检查 PPAR gamma 2 转录的 C/EBP 依赖性。在 UMR106 细胞中，C/EBP α 和 C/EBP δ 表达载体均将 PPAR γ 2 启动子/荧光素酶表达载体的转录激活了 5-6 倍。同时转染 C/EBP 同源蛋白 (CHOP)（也称为生长停滞 DNA 损伤蛋白 153 或 gadd153）以浓度依赖性方式抑制这种 C/EBP 依赖性激活。已知 CHOP 蛋白与其他 C/EBP 蛋白异二聚化，形成转录失活复合物。 PPAR gamma 2启动子内-340 bp和-327 bp处的两个C/EBP DNA识别元件的突变降低了C/EBP α和C/EBP δ的诱导作用。这些发现表明 C/EBP 家族中的蛋白质直接调节 PPAR gamma 2 启动子的转录。 (C) 1997 年学术出版社。
The CCAAT/enhancer binding proteins (C/EBPs) and the peroxisome proliferator-activated receptors (PPARs) together regulate adipogenesis. The current work, uses co-transfection studies to examine the C/EBP dependence of PPAR gamma 2 transcription. Both C/EBP alpha and C/EBP delta expression vectors activated transcription from a PPAR gamma 2 promoter/luciferase expression vector by 5-6 fold in UMR106 cells. The simultaneous transfection of the C/EBP homologous protein (CHOP) (also known as growth arrest DNA damage protein 153 or gadd153) inhibited this C/EBP-dependent activation in a concentration dependent manner. The CHOP protein is known to heterodimerize with other C/EBP proteins to form transcriptionally inactive complexes. Mutation of the two C/EBP DNA recognition elements at -340 bp and -327 bp within the PPAR gamma 2 promoter reduced the inductive effects of both C/EBP alpha and C/EBP delta. These findings demonstrate that proteins within the C/EBP family directly modulate transcription from the PPAR gamma 2 promoter. (C) 1997 Academic Press.