Recombinant interferon-alpha, -beta, and -gamma enhance the proliferative response of human B cells.
Recombinant interferon-alpha, -beta, and -gamma enhance the proliferative response of human B cells.
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DOI：
10.4049/jimmunol.139.3.761
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发表时间：
1987-08
影响因子：
4.4
通讯作者：
K. Morikawa;H. Kubagawa;T. Suzuki;M. Cooper
中科院分区：
文献类型：
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作者：
K. Morikawa;H. Kubagawa;T. Suzuki;M. Cooper
检测重组干扰素（IFN-α、IFN-β和IFN-γ）对B细胞活化的影响。通过荧光激活细胞分选从人血液样品中分离相对小的IgM+ B细胞，并将其用作靶细胞。虽然干扰素本身是非促有丝分裂的，但每种干扰素都能增强促有丝分裂抗μ单克隆抗体诱导的增殖反应，IFN-β通常表现出最大的增强作用，IFN-γ表现出最小的增强作用。用干扰素预处理引发静息B细胞以响应抗μ抗体DA 4而经历增强的DNA合成。相反，抗mu预处理，随后IFN处理，不诱导B细胞进入S期。时程分析表明，IFN可以增强抗mu反应，即使在最后24小时的3天的培养间隔。IFN-γ加IFN-α或IFN-β的组合在抗μ应答中是协同的，而IFN-α加IFN-β的组合不是。这些数据表明，由淋巴细胞（IFN-γ）和非淋巴炎症细胞（IFN-α和-β）产生的干扰素可以通过不同的机制增强B细胞的生长。
Recombinant interferons (IFN-alpha, -beta, and -gamma) were examined for their effects on B cell activation. Relatively small IgM+ B cells from human blood samples were isolated by fluorescence-activated cell sorting and were used as target cells. Although the interferons themselves were nonmitogenic, each enhanced the proliferative response induced by a mitogenic anti-mu monoclonal antibody, with IFN-beta usually showing the greatest enhancement and IFN-gamma the least. Pretreatment with the interferons primed resting B cells to undergo enhanced DNA synthesis in response to the anti-mu antibody DA4. Conversely, anti-mu pretreatment, followed by IFN treatment, did not induce B cells to enter the S phase. Time-course analysis revealed that IFN could augment the anti-mu response even when added as late as the final 24 hr of a 3-day culture interval. Combinations of IFN-gamma plus IFN-alpha or -beta were synergistic in the anti-mu response, whereas the IFN-alpha plus IFN-beta combination was not. The data suggest that interferons produced by both lymphocytes (IFN-gamma) and nonlymphoid inflammatory cells (IFN-alpha and -beta) can enhance B cell growth via different mechanisms.