Generation of Toxoplasma gondii and Hammondia hammondi Oocysts and Purification of Their Sporozoites for Downstream Manipulation.
Generation of Toxoplasma gondii and Hammondia hammondi Oocysts and Purification of Their Sporozoites for Downstream Manipulation.
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DOI：
10.1007/978-1-4939-9857-9_4
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Dubey JP
中科院分区：
文献类型：
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作者：
Sokol SL;Wong ZS;Boyle JP;Dubey JP
弓形虫速殖子和缓殖子由于易于培养而在实验室中被广泛研究。相反，卵囊和其中的子孢子更难以处理，因为它们的产生需要猫感染，并且分离子孢子需要费力的脱囊程序。此外，一些寄生虫物种如Hammondia hammondi是专性异源的，需要通过猫来完成生命周期。毫无疑问，研究这一重要的生命周期阶段具有很大的价值，因此我们在这里详细描述了我们实验室用于产生和分离T. gondii和H. Hammondi卵囊，并将其内的子孢子脱囊和纯化用于下游实验应用。
Toxoplasma gondii tachyzoites and bradyzoites are studied extensively in the laboratory due to the ease with which they can be cultured. In contrast oocysts and the sporozoites within them are more difficult to work with, in that cat infections are required for their generation and isolating sporozoites requires a laborious excystation procedure. More over some parasite species such as Hammondia hammondi are obligately heteroxenous and require passage through a cat for completion of the life cycle. There is no debate that there is great value in studying this important life cycle stage, and so we present here a detailed description of the current protocols used in our laboratories to generate and isolate T. gondii and H. hammondi oocysts, and to excyst and purify the sporozoites within them for use in downstream experimental applications.