Columnar alterations of NADH fluorescence during hypoxia-ischemia in immature rat brain.
Columnar alterations of NADH fluorescence during hypoxia-ischemia in immature rat brain.
复制标题
未成熟大鼠脑缺氧缺血期间 NADH 荧光的柱状变化。
DOI：
10.1038/jcbfm.1982.22
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Brierley,JB
中科院分区：
文献类型：
--
作者：
Welsh,FA;Vannucci,RC;Brierley,JB
采用单侧颈总动脉结扎结合全身缺氧（8%O2）的方法，建立新生7天大鼠脑缺血缺氧模型。高能量磷酸盐的水平，这只是略有改变，在对侧半球，几乎耗尽在同侧半球在3小时缺氧损伤。在缺氧持续1至3小时的情况下，皮质NADH荧光的柱状改变发生在与先前证实的组织学损伤相同的位置和区域模式中（Rice等人，1981年）。在区域表现出列的NADH荧光，有没有证据的柱状减少的高能量磷酸盐的ATP和磷酸肌酸的水平几乎为零。从3小时的缺氧恢复伴随着部分和区域异质性恢复ATP同侧半球内。在恢复期内未检测到NADH荧光的柱状变化;相反，与对侧半球相比，高能磷酸盐恢复受损的区域表现出弥漫性减少的NADH荧光。抑制的NADH荧光和耗尽的ATP之间的相关性，目前作为皮质柱在缺氧和恢复过程中作为较大的区域，表明减少形成的NADH可能限制了高能量磷酸盐的再合成。
Cerebral hypoxia-ischemia was produced in 7-day postnatal rats by unilateral carotid artery ligation combined with systemic hypoxia (8% O2). Levels of high energy phosphates, which were only slightly altered in the contralateral hemisphere, were nearly depleted in the ipsilateral hemisphere during the 3-h hypoxic insult. With hypoxia of between 1 and 3 hours' duration, columnar alterations of cortical NADH fluorescence occurred in the same location and regional pattern as did histologic damage demonstrated previously (Rice et al., 1981). In regions exhibiting columns of NADH fluorescence, there was no evidence of a columnar reduction of high energy phosphates as levels of ATP and phosphocreatine were nearly zero.Recovery from 3 h of hypoxia was accompanied by partial and regionally heterogeneous restoration of ATP within the ipsilateral hemisphere. Columnar variations of NADH fluorescence were not detected in the recovery period; rather, regions with impaired restitution of high energy phosphates exhibited NADH fluorescence that was diminished diffusely compared to the contralateral hemisphere. The correlation between depressed NADH fluorescence and depleted ATP, present as cortical columns during hypoxia and as larger regions during recovery, suggests that decreased formation of NADH may be limiting the resynthesis of high energy phosphates.