ANALYSIS OF A HISTONE H2A VARIANT FROM FISSION YEAST - EVIDENCE FOR A ROLE IN CHROMOSOME STABILITY
ANALYSIS OF A HISTONE H2A VARIANT FROM FISSION YEAST - EVIDENCE FOR A ROLE IN CHROMOSOME STABILITY
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DOI：
10.1007/bf00282226
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发表时间：
1994-12-01
期刊：
影响因子：
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通讯作者：
NURSE, P
中科院分区：
文献类型：
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作者：
CARR, AM;DORRINGTON, SM;NURSE, P
我们已经从裂殖酵母粟酒裂殖酵母中分离并鉴定了pht 1基因。预测的翻译产物的序列揭示了与H2A.F/Z组蛋白变体蛋白家族的惊人相似性，该家族已在多种不同的生物体中发现。pht 1基因位点缺失的细胞生长缓慢，表现出改变的集落形态，对热休克的抗性增加，并显示出S.粟酒属微型染色体。我们建议，pht 1基因编码的组蛋白H2 A变体是重要的染色体结构和功能，可能包括在有丝分裂过程中控制染色体分离的保真度的作用。
We have isolated and characterised the pht1 gene from the fission yeast Schizosaccharomyces pombe. The sequence of the predicted translation product has revealed a striking similarity to the family of H2A.F/Z histone variant proteins, which have been found in a variety of different organisms. Cells deleted for the pht1 gene locus grow slowly, exhibit an altered colony morphology, increased resistance to heat shock and show a significant decrease in the fidelity of segregation of an S. pombe minichromosome. We propose that the histone H2A variant encoded by the pht1 gene is important for chromosomal structure and function, possibly including a role in controlling the fidelity of chromosomal segregation during mitosis.