Tryptophan Prevents the Development of Non-Alcoholic Fatty Liver Disease.
Tryptophan Prevents the Development of Non-Alcoholic Fatty Liver Disease.
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DOI：
10.2147/dmso.s444278
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发表时间：
2023
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影响因子：
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中科院分区：
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本研究的主要目的是研究色氨酸对高热量饮食（HCD）引起的肝脏组织形态学和生化异常的保护作用，以及其使线粒体功能正常化的能力，从而预防非酒精性脂肪性肝病（NAFLD）的发展。本研究是在实验开始时3个月大的雄性Wistar大鼠中进行的。对照组（ⅰ组）饲喂标准日粮。第二组实验动物连续12周饲喂过量脂肪（45%）和碳水化合物（31%）的饲粮。第三组实验动物除给予HCD外，还给予l -色氨酸80 mg/kg体重的剂量。使用生理学、形态学、组织形态计量学、生化和生物物理研究方法评估NAFLD的存在、功能活性、生理再生以及肝实质和结缔组织的状态。HCD诱导NAFLD的发展，其特征是肝脏重量增加，肝细胞肥大，肝组织中脂质、胆固醇和甘油三酯浓度升高。肥胖大鼠肝脏丙氨酸转氨酶活性升高也证实肝细胞受损。添加到饮食中的色氨酸通过减少脂肪积累和违反生物电特性降低了NAFLD的严重程度，并阻止了线粒体ATP合成的减少。添加色氨酸可以对肝脏产生潜在的积极作用，减轻HCD引起的结构、生化、线粒体和生物电损伤的严重程度。
The main aim of this research is to study the protective effects of tryptophan on the histomorphological and biochemical abnormalities in the liver caused by a high-calorie diet (HCD), as well as its ability to normalize mitochondrial functions in order to prevent the development of non-alcoholic fatty liver disease (NAFLD). The study was conducted in male Wistar rats aged 3 months at the start of the experiment. Control animals (group I) were fed a standard diet. Group II experimental animals were fed a diet with an excess of fat (45%) and carbohydrates (31%) for 12 weeks. Group III experimental animals also received L-tryptophan at a dose of 80 mg/kg body weight in addition to the HCD. The presence of NAFLD, functional activity, physiological regeneration, and the state of the liver parenchyma and connective tissue were assessed using physiological, morphological, histo-morphometric, biochemical, and biophysical research methods. HCD induced the development of NAFLD, which is characterized by an increase in liver weight, hypertrophy of hepatocytes and an increase in the concentration of lipids, cholesterol and triglycerides in liver tissue. Increased alanine aminotransferase activity in the liver of obese rats also confirm hepatocytes damage. Tryptophan added to the diet lowered the severity of NAFLD by reducing fat accumulation and violations of bioelectric properties, and prevented a decrease in mitochondrial ATP synthesis. The addition of tryptophan can have a potential positive effect on the liver, reducing the severity of structural, biochemical, mitochondrial and bioelectric damage caused by HCD.
影响因子：
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作者：
Shchelykalina SP;Nikolaev DV;Kolesnikov VA;Korostylev KA;Starunova OA
通讯作者：
Starunova OA