Hypoxia-induced alteration of RNA modifications in the mouse testis and spermA
Hypoxia-induced alteration of RNA modifications in the mouse testis and spermA
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DOI：
10.1093/biolre/ioab142
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发表时间：
2021-07-23
影响因子：
3.6
通讯作者：
Tan, Yi
中科院分区：
文献类型：
--
作者：
He, Tong;Guo, Huanping;Tan, Yi
高原低压缺氧作为一种极端环境，对人体健康有着严重的危害。研究表明，匆忙进入高原可能会降低男性生育能力，并表现在精子数量减少和精子活力减弱。RNA修饰对环境变化敏感，最近已成为男性精子发生和代际表观遗传中的新型转录后调节因子。在本研究中，我们建立了一个小鼠缺氧模型，模拟在海拔5500米的环境中35天，这导致精子发生受损，精子数量减少，精子畸形率增加。使用这种缺氧模型，我们进一步应用了我们最近开发的基于液相色谱串联质谱的高通量RNA修饰定量平台，该平台具有同时检测25种RNA修饰的能力。结果显示，低氧条件下，睾丸（6种）和成熟精子（11种）的RNA修饰特征发生了改变，其中4种类型（Am、Gm、m（7）G和m（2）（2）G）发生了重叠。我们的数据首次绘制了RNA修饰谱的特征，并全面分析了小鼠睾丸和精子在缺氧模型下RNA修饰水平的变化。这些数据可能是高度相关的人类条件下，类似的缺氧environment.Summary EQUIPMENT使用高通量LC-MS/MS，我们的研究揭示了改变签名的RNA修饰在小鼠睾丸和精子缺氧模型下。这些数据可能与类似缺氧环境中的人体状况密切相关。
Hypobaric hypoxia as an extreme environment in a plateau may have deleterious effects on human health. Studies have indicated that rush entry into a plateau may reduce male fertility and manifest in decreased sperm counts and weakened sperm motility. RNA modifications are sensitive to environmental changes and have recently emerged as novel post-transcriptional regulators in male spermatogenesis and intergenerational epigenetic inheritance. In the present study, we generated a mouse hypoxia model simulating the environment of 5500 m in altitude for 35 days, which led to compromised spermatogenesis, decreased sperm counts, and an increased sperm deformation rate. Using this hypoxia model, we further applied our recently developed high-throughput RNA modification quantification platform based on liquid chromatography with tandem mass spectrometry, which exhibited the capacity to simultaneously examine 25 types of RNA modifications. Our results revealed an altered sperm RNA modifications signature in the testis (6 types) and mature sperm (11 types) under the hypoxia model, with 4 types showing overlap (Am, Gm, m(7)G, and m(2)(2)G). Our data first drew the signature of RNA modification profiles and comprehensively analyzed the alteration of RNA modification levels in mouse testis and sperm under a mouse hypoxia model. These data may be highly related to human conditions under a similar hypoxia environment.Summary sentenceUsing high-throughput LC-MS/MS, our study revealed an altered signature of RNA modifications in the mouse testis and sperm under a hypoxia model. These data may be strongly related to human conditions in a similar hypoxia environment.