AMINO-TERMINAL FRAGMENTS OF ESCHERICHIA-COLI LAC REPRESSOR BIND TO DNA
AMINO-TERMINAL FRAGMENTS OF ESCHERICHIA-COLI LAC REPRESSOR BIND TO DNA
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DOI：
10.1038/269668a0
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发表时间：
1977-01-01
期刊：
影响因子：
64.8
通讯作者：
WEBER, K
中科院分区：
文献类型：
--
作者：
JOVIN, TM;GEISLER, N;WEBER, K
通过选择性胰蛋白酶裂解天然lac抑制因子获得的N[氨基]末端片段（残基1-51和1-59）保留了结合DNA的能力。这些片段（头饰）是单体的，形成类似于四聚体抑制因子与非操作子DNA的复合物，但它们对操作子序列没有表现出高特异性。通过过滤结合试验和溶液中吸收、圆二色性和荧光测量证明了DNA结合。
The N[amino]-terminal fragments (residues 1-51 and 1-59) obtained by selective tryptic cleavage of native lac repressor retain the ability to bind DNA. These fragments (headpieces) are monomeric and form complexes which resemble those of tetrameric repressor with non-operator DNA, but they do not show the high specificity of repressor for operator sequences. The DNA binding was demonstrated by filter-binding assay and in solution using absorption, circular dichroism and fluorescence measurements.