High-resolution Imaging of pH in Alkaline Sediments and Water Based on a New Rapid Response Fluorescent Planar Optode.
High-resolution Imaging of pH in Alkaline Sediments and Water Based on a New Rapid Response Fluorescent Planar Optode.
复制标题
基于新型快速响应荧光平面光极的碱性沉积物和水中 pH 值高分辨率成像
DOI：
10.1038/srep26417
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发表时间：
2016-05-20
影响因子：
4.6
通讯作者：
Zhang C
中科院分区：
文献类型：
--
作者：
Han C;Yao L;Xu D;Xie X;Zhang C
开发了一种新的双荧光团光学传感器结合一个强大的RGB参考方法的二维（2D）pH成像在碱性沉积物和水。pH传感器膜由质子可渗透聚合物（PVC）组成，其中包埋了具有不同pH灵敏度和发射颜色的两种染料：（1）氯苯亚氨基丙烯基苯胺（CPIPA）和（2）香豆素染料Macrolex®荧光黄10 GN（MFY-10 GN）。校准实验揭示了典型的sigmoid函数和温度依赖性。该传感器在pH 7.5 ~ 10.5的碱性工作范围内具有高灵敏度和快速响应的特点。当离子强度（IS）<0.1 M时，发现对淡水的交叉敏感性可忽略不计。该传感器的空间分辨率约为22 μm，在pH 7.0 ~ 9.0范围内响应时间<120 s。利用pH微电极验证了该传感器的可行性。文中还给出了一个在淡水沉积物和与苦草属植物光合作用有关的水体中获得pH图像的例子，表明该传感器具有很好的现场应用前景。
A new dual-lumophore optical sensor combined with a robust RGB referencing method was developed for two-dimensional (2D) pH imaging in alkaline sediments and water. The pH sensor film consisted of a proton-permeable polymer (PVC) in which two dyes with different pH sensitivities and emission colors: (1) chloro phenyl imino propenyl aniline (CPIPA) and (2) the coumarin dye Macrolex®fluorescence yellow 10 GN (MFY-10 GN) were entrapped. Calibration experiments revealed the typical sigmoid function and temperature dependencies. This sensor featured high sensitivity and fast response over the alkaline working ranges from pH 7.5 to pH 10.5. Cross-sensitivity towards ionic strength (IS) was found to be negligible for freshwater when IS <0.1 M. The sensor had a spatial resolution of approximately 22 μm and aresponse time of <120 s when going from pH 7.0 to 9.0. The feasibility of the sensor was demonstrated using the pH microelectrode. An example of pH image obtained in the natrual freshwater sediment and water associated with the photosynthesis ofVallisneria spiralspecies was also presented, suggesting that the sensor held great promise for the field applications.