60 kDa Lysophospholipase, a New Sgk1 Molecular Partner Involved in the Regulation of ENaC
60 kDa Lysophospholipase, a New Sgk1 Molecular Partner Involved in the Regulation of ENaC
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DOI：
10.1159/000322326
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发表时间：
2010-01-01
影响因子：
--
通讯作者：
Perrotti, Nicola
中科院分区：
文献类型：
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作者：
Menniti, Miranda;Iuliano, Rodolfo;Perrotti, Nicola
The serum- and glucocorticoid-regulated kinase (Sgk1) is essential for hormonal regulation of ENaC-mediated sodium transport and is involved in the transduction of growth-factor-dependent cell survival and proliferation. The identification of molecular partners for Sgk1 is crucial for the understanding of its mechanisms of action. We performed a yeast two-hybrid screening based on a human kidney cDNA library to identify molecular partners of Sgk1. As a result the screening revealed a specific interaction between Sgk1 and a 60 kDa Lysophospholipase (LysoLP). LysoLP is a poorly characterized enzyme that, based on sequence analysis, might possess lysophospholipase and asparaginase activities. We demonstrate that LysoLP has indeed a lysophospholipase activity and affects metabolic functions related to cell proliferation and regulation of membrane channels. Moreover we demonstrate in the Xenopus oocyte expression system that LysoLP downregulates basal and Sgk1-dependent ENaC activity. In conclusion LysoLP may represent a new player in the regulation of ENaC and Sgk1-dependent signaling. Copyright (C) 2010 S. Karger AG, Basel
