Induction of in vitro differentiation and immunoglobulin synthesis of human leukemic B lymphocytes.
Induction of in vitro differentiation and immunoglobulin synthesis of human leukemic B lymphocytes.
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DOI：
10.1084/jem.148.6.1570
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发表时间：
1978-12-01
期刊：
影响因子：
--
通讯作者：
Harris SR
中科院分区：
文献类型：
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作者：
Fu SM;Chiorazzi N;Kunkel HG;Halper JP;Harris SR
成功地对2例慢性淋巴细胞白血病患者的白血病淋巴细胞进行了体外诱导分化和免疫球蛋白合成。分离的白血病B细胞在存在或不存在自体T细胞的情况下，培养的浆细胞很少，特异性Ig分泌也很少。高达30%的白血病B细胞成熟为浆细胞，当正常人的T细胞加入到这些白血病B细胞的培养中时，特异性Ig合成增加了32倍。在两例中的一例中，在商陆有丝分裂原存在的情况下，自体T细胞能够诱导超过50%的白血病B细胞进一步分化为浆细胞。只有来自体内有轻微分化证据的病例的白血病细胞，才能在体外显著加速分化。没有证据表明这些患者体内存在过多的抑制性T细胞。然而，在同种异体效应辅助因子的产生中发现了一种T细胞缺陷。这一缺陷可能是体内白血病成熟率降低的原因。
Successful induction of in vitro differentiation and immunoglobulin synthesis of the leukemic lymphocytes was carried out in two cases of chronic lymphocytic leukemia. Few plasma cells and little specific Ig secretion were detected in the cultures of isolated leukemic B cells in either the presence or the absence of autologous T cells. Up to 30% of the leukemic B cells matured to plasma cells, and a 32-fold increase in specific Ig synthesis was observed when T cells from normal individuals were added to the cultures of these leukemic B cells. In one of the two cases, autologous T cells were able to induce greater than 50% of the leukemic B cells to differentiate further to plasma cells in the presence of pokeweed mitogen. This markedly accelerated in vitro differentiation was only achieved with leukemic cells from cases in which there was evidence of slight differentiation in vivo. No evidence could be obtained for excessive suppressor T cells in these patients. However, a T-cell defect in the generation of allogeneic effect helper factors was identified. This defect may be responsible for the reduced rate of leukemic maturation in vivo.