Development of a fully integrated microfluidic system for sensing infectious viral disease
Development of a fully integrated microfluidic system for sensing infectious viral disease
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DOI：
10.1002/elps.200700823
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发表时间：
2008-07-01
期刊：
影响因子：
2.9
通讯作者：
Lee, Sang Yup
中科院分区：
文献类型：
--
作者：
Huh, Yun Sulk;Park, Tae Jung;Lee, Sang Yup
开发了一种利用磁力的主动微混合器系统，并对其促进两种以上流体混合的能力进行了测试。对活性微混合器在含染料的水-水体系中的混合性能进行了评价。将多种功能模块集成到单个芯片上，实现细胞裂解、样品制备、细胞内分子纯化及后续分析，开发出完整的分析微流控系统。在将细胞样品和裂解溶液装入混合室后，集成的微流控装置通过旋转微磁盘和使用特氟龙涂层疏水膜作为微阀控制混合时间来实现有效的细胞破坏。流入后，使用丙烯酰胺（AAm）功能化的SPE从细胞裂解液样品中分离细胞碎片和受污染的蛋白质。流入的含有金结合多肽（GBP）融合蛋白的部分纯化细胞裂解液样品通过其金属结合亲和力与金微图案结合。gbp融合方法允许将生物活性形式的蛋白质固定在金表面上，而不需要进行适合研究抗原-抗体相互作用的表面修饰。它被用于检测严重急性呼吸系统综合症（SARS），一种传染性病毒疾病，作为一个例子。
An active micromixer system utilizing the magnetic force was developed and examined for its ability to facilitate the mixing of more than two fluid flows. The mixing performance of the active micromixer was evaluated in aqueous-aqueous systems including dyes for visual observation. A complete analytical microfluidic system was developed by integrating various functional modules into a single chip, thus allowing cell lysis, sample preparation, purification of intracellular molecules, and subsequent analysis. Upon loading the cell samples and lysis solution into the mixing chamber, the integrated microfluidic device allows efficient cell disruption by rotation of a micromagnetic disk and control of mixing time using the Teflon-coated hydrophobic film as a microvalve. This inflow is followed by separating the cell debris and contaminated proteins from the cell lysate sample using the acrylamide (AAm)-functionalized SPE. The inflow of partially purified cell lysate sample containing the gold binding polypeptide (GBP)-fusion protein was bound onto the gold micropatterns by means of its metal binding affinity. The GBP-fusion method allows immobilization of proteins in bioactive forms onto the gold surface without surface modification suitable for studying antigen-antibody interaction. It was used for the detection of severe acute respiratory syndrome (SARS), an infectious viral disease, as an example case.