INDUCTION OF INTERFERON-ALPHA BY INTERFERON-BETA, BUT NOT OF INTERFERON-BETA BY INTERFERON-ALPHA, IN THE MOUSE
INDUCTION OF INTERFERON-ALPHA BY INTERFERON-BETA, BUT NOT OF INTERFERON-BETA BY INTERFERON-ALPHA, IN THE MOUSE
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DOI：
10.1016/0042-6822(90)90227-i
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发表时间：
1990-05-01
期刊：
影响因子：
3.7
通讯作者：
IWAKURA, Y
中科院分区：
文献类型：
--
作者：
ASANO, M;HAYASHI, M;IWAKURA, Y
携带额外小鼠IFN-β的转基因小鼠产生的干扰素(IFN)的抗原性。用 Cd2+ 诱导后，检查了小鼠金属硫蛋白-I 增强子-启动子控制下的基因。出乎意料的是，IFN-α。除 IFN-β 外。在血清中检测到。 IFN-α的诱导。当重组小鼠IFN-β时也观察到。被注射到正常小鼠体内。 IFN-α。施用IFN-β后6-10小时在循环中首次检测到IFN-β，12小时后，IFN-α。成为血清干扰素的主要成分。另一方面，当IFN-α。注射后，没有产生IFN-β。被观察到。 IFN-.alpha 特异性的信使 RNA。和内源性IFN-β。在脾脏中检测到 IFN-β 的量。 mRNA远低于IFN-α。 mRNA。在其他器官中未检测到这些 mRNA，包括存在外源 IFN-β 的肝脏。基因显着表达。这些观察表明IFN-α的表达。可由IFN-β诱导。在小鼠中，脾脏被认为是主要的生产场所。讨论了可能的诱导机制。
The antigenicity of the interferon (IFN) produced in transgenic mice carrying an extra mouse IFN-.beta. gene under the control of mouse metallothionein-I enhancer-promoter was examined after induction with Cd2+. Unexpectedly, IFN-.alpha. in addition to IFN-.beta. was detected in the serum. Induction of IFN-.alpha. was also observed when recombinant mouse IFN-.beta. was injected into normal mice. IFN-.alpha. was first detected in the circulation 6-10 hr after the administration of IFN-.beta., and after 12 hr, IFN-.alpha. became the major component of serum IFN. On the other hand, when IFN-.alpha. was injected, no production of IFN-.beta. was observed. Messenger RNAs specific for IFN-.alpha. and endogenous IFN-.beta. were detected in the spleen, though the amount of IFN-.beta. mRNA was much less than that of IFN-.alpha. mRNA. These mRNAs were not detected in other organs including the liver where exogenous IFN-.beta. gene was markedly expressed. These observations showed that the expresion of IFN-.alpha. is inducible by IFN-.beta. in the mouse, and the spleen was suggested to be the main site of production. Possible mechanisms of the induction are discussed.