DETECTION OF MYCOBACTERIUM-TUBERCULOSIS IN CLINICAL SPECIMENS BY POLYMERASE CHAIN-REACTION AND GEN-PROBE AMPLIFIED MYCOBACTERIUM-TUBERCULOSIS DIRECT TEST
DETECTION OF MYCOBACTERIUM-TUBERCULOSIS IN CLINICAL SPECIMENS BY POLYMERASE CHAIN-REACTION AND GEN-PROBE AMPLIFIED MYCOBACTERIUM-TUBERCULOSIS DIRECT TEST
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DOI：
10.1128/jcm.31.12.3270-3274.1993
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发表时间：
1993-12-01
影响因子：
9.4
通讯作者：
GOTO, S
中科院分区：
文献类型：
--
作者：
ABE, C;HIRANO, K;GOTO, S
采用基于结核分枝杆菌重复序列（IS 986）的寡核苷酸作为引物的聚合酶链反应（PCR）和Gen-Probe扩增结核分枝杆菌直接试验（MTD），该试验结合了结核分枝杆菌（M.结核分枝杆菌rRNA扩增方法与杂交保护试验的形式，用于检测M。临床样本中的结核病。这两种基于核酸扩增的检测系统对培养的M.结核病的细胞少于10个细胞/反应。两种检测系统共检测了135份痰液标本。建立了检测M.结核病的总阳性率分别为84.2%（32/38）和91.9%（34/37），而涂片法和液体培养基MB-Check培养法的总阳性率分别为71.9%（23/32）和96.9%（31/32）。两种方法中所用的样品制备程序不同。虽然PCR和MTD的灵敏度与MB-Check系统培养的灵敏度相似，但这两种基于核酸扩增的方法在快速检测M.结核感染，而不需要长时间的M.结核
The polymerase chain reaction (PCR) using oligonucleotides based on the repetitive sequence (IS986) of Mycobacterium tuberculosis as a primer and the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test (MTD), which combines an M. tuberculosis rRNA amplification method with the hybridization protection assay format, were evaluated for detection of M. tuberculosis in clinical samples. The detection limits of these two assay systems based on nucleic acid amplification for cultured M. tuberculosis were less than 10 cells per reaction. A total of 135 sputum specimens were examined by the two assay systems. The PCR and the MTD systems for detection of M. tuberculosis gave overall positivity rates of 84.2% (32 of 38) and 91.9% (34 of 37), respectively, as compared with 71.9% (23 of 32) by smear and 96.9% (31 of 32) by culture in the liquid medium MB-Check. Procedures for sample preparation used in the two methods were different. Although the sensitivities of the PCR and MTD appeared to be similar to that of culture with the MB-Check system, the two methods based on nucleic acid amplification should be very useful for rapid detection of M. tuberculosis infections without the long time required for culture of M. tuberculosis.