Identification of crucial factors involved in Cynoglossus semilaevis sexual size dimorphism by GWAS and demonstration of zbed1 regulatory network by DAP-seq
Identification of crucial factors involved in Cynoglossus semilaevis sexual size dimorphism by GWAS and demonstration of zbed1 regulatory network by DAP-seq
复制标题
通过 GWAS 鉴定参与半滑舌鱼性别大小二态性的关键因素并通过 DAP-seq 演示 zbed1 调控网络
DOI：
10.1016/j.ygeno.2022.110376
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发表时间：
2022
期刊：
影响因子：
4.4
通讯作者：
Songlin Chen
中科院分区：
文献类型：
--
作者：
Na Wang;Jin Gao;Yang Liu;Rui Shi;Songlin Chen
性别大小二型性(SSD)，即雌性和雄性表现出不同的身体大小，在动物中被广泛记录。为探讨影响半滑舌鲨性别偏向的关键调节因素，本研究对350名女性和59名男性进行了性别差异分析。筛选到20个SNPs和25个基因，其中zbed1、nsd3、cdc45、kll29、Smad4与-log(P)>；7主要定位在性染色体上。染色体W连锁基因zbed1引起了特别的关注，因为它是细胞增殖的主键。因此，用DAP-SEQ方法探索了半滑链藻中zbed1的调控网络，并在雌性大脑中发现了1352个峰。此外，zbed1还可能调控河马信号通路、细胞周期、翻译和PI3K-Akt信号通路。这些发现确定了与半滑舌藻中雌性偏向的SSD相关的关键SNPs和基因，也为鱼类物种中的zbed1调控网络提供了第一次全基因组调查。
Sexual size dimorphism (SSD), whereby females and males exhibit different body sizes, are widely documented in animals. To explore crucial regulators implicated in female-biased SSD of Chinese tongue sole (Cynoglossus semilaevis), GWAS was conducted on 350 females and 59 males. Twenty SNPs and 25 genes including zbed1, nsd3, cdc45, klhl29, and smad4 with -log(p) > 7 were screened, mainly mapping to sex chromosome. The chromosome W-linked gene zbed1 attracted particular attention because it is a master key for cell proliferation. Thus, the regulatory network of zbed1 in C. semilaevis was explored by DAP-seq and 1352 peaks were discovered in the female brain. Moreover, zbed1 potentially regulated hippo signaling pathway, cell cycle, translation, and PI3k-Akt signaling pathway in C. semilaevis. These findings identify crucial SNPs and genes associated with female-biased SSD in C. semilaevis, also provide the first genome-wide survey for the zbed1 regulatory network in fish species.