Expression profile of the sex determination gene doublesex in a gynandromorph of bumblebee, Bombus ignitus.
Expression profile of the sex determination gene doublesex in a gynandromorph of bumblebee, Bombus ignitus.
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熊蜂雌雄同体中性别决定基因 doublesex 的表达谱。
DOI：
10.1007/s00114-016-1342-7
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发表时间：
2016
影响因子：
--
通讯作者：
Ono M.
中科院分区：
文献类型：
--
作者：
Ugajin A;Matsuo K;Kubo R;Sasaki T;Ono M.
雌雄同体的现象在许多昆虫目中都有，包括Hyattera。然而，在大多数情况下，只有外部形态和行为方面进行了研究。我们发现熊蜂的雌雄同体，熊蜂，表现出几乎双边分布的外部性特征，男性字符观察到的左侧和右侧的女性字符。这个人从未对新女王表现出性行为。头部解剖显示，它有双侧二形唇腺，只有左侧唇腺发育良好，并合成雄性特异性信息素成分。而雌雄同体的腹部则有一个产卵器和一对卵巢，表明其具有统一的雌性生殖系统。此外，我们的特点是几个内部器官的雌雄同体的分子生物学方法。性别决定基因，doublaex，在大脑中的表达分析，脂肪体，后肠，和卵巢的gynandromorph揭示了男性型的表达模式，专门在左脑半球和一致的女性型的表达在其他组织。这些发现清楚地表明，在雌雄同体的外部特征和内部器官之间的性别不一致。利用微卫星标记进行遗传分析的结果表明，这一个体由遗传雄性和雌性型组织。
Gynandromorphy that has both male and female features is known in many insect orders, including Hymenoptera. In most cases, however, only external morphology and behavioral aspects have been studied. We found a gynandromorph of bumblebee,Bombus ignitus, that showed almost bilateral distribution of external sexual traits, with male characters observed on the left side and female characters on the right side. This individual never exhibited sexual behavior toward new queens. The dissection of the head part showed that it had bilaterally dimorphic labial glands, only the left of which was well developed and synthesized male-specific pheromone components. In contrast, the gynandromorph possessed an ovipositor and a pair of ovaries in the abdominal part, suggesting that it had a uniformly female reproductive system. Furthermore, we characterized several internal organs of the gynandromorph by a molecular biological approach. The expression analyses of a sex determination gene,doublesex, in the brain, the fat bodies, the hindgut, and the ovaries of the gynandromorph revealed a male-type expression pattern exclusively in the left brain hemisphere and consistent female-type expression in other tissues. These findings clearly indicate the sexual discordance between external traits and internal organs in the gynandromorph. The results of genetic analyses using microsatellite markers suggested that this individual consisted of both genetically male- and female-type tissues.