DAUGHTER 3T3 CELLS - ARE THEY MIRROR IMAGES OF EACH OTHER
DAUGHTER 3T3 CELLS - ARE THEY MIRROR IMAGES OF EACH OTHER
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DOI：
10.1083/jcb.72.3.595
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发表时间：
1977-01-01
影响因子：
7.8
通讯作者：
ALBRECHTBUEHLER, G
中科院分区：
文献类型：
--
作者：
ALBRECHTBUEHLER, G
使用一种新的技术来可视化移动的3 T3 [小鼠成纤维细胞]细胞的轨道，并结合它与可视化肌动蛋白含有微丝束的间接免疫荧光，实验表明，30-40%的对的女儿3 T3小鼠成纤维细胞在非克隆培养物具有镜像对称肌动蛋白束模式。分离的子细胞之间的角度是. apprx。90.degree.或180度。并且似乎与某些含肌动蛋白束的方向有关。大约40%的单独移动的子细胞，不与任何其他细胞在培养中碰撞进行方向的变化，在镜像对称的方式。两个子细胞几乎同时进入下一次有丝分裂。肌动蛋白束的模式、分离的角度、间期的主要方向变化和下一次有丝分裂的时间可以由亲本细胞预先确定。
Using a new technique to visualize the tracks of moving 3T3 [mouse fibroblast] cells and combining it with the visualization of actin-containing microfilament bundles by indirect immunofluorescence, experiments are presented which suggest that 30-40% of the pairs of daughter 3T3 mouse fibroblasts in noncloned cultures have mirror symmetrical actin-bundle patterns. The angle between separating daughter cells is .apprx. 90.degree. or 180.degree. and seems related to the directions of certain actin-containing bundles. Approximately 40% of separately moving daughter cells which did not collide with any other cell in the culture performed directional changes in a mirror symmetrical way. Both daughter cells entered the next mitosis at approximately the same time. The actin-bundle pattern, the angle of separation, major directional changes during interphase and the time of the next mitosis may be predetermined by the parental cell.