A P-31 AND H-1-NMR INVESTIGATION IN-VITRO OF NORMAL AND ABNORMAL HUMAN LIVER
A P-31 AND H-1-NMR INVESTIGATION IN-VITRO OF NORMAL AND ABNORMAL HUMAN LIVER
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DOI：
10.1016/0925-4439(93)90124-j
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发表时间：
1993-11-25
期刊：
影响因子：
--
通讯作者：
URENJAK, J
中科院分区：
文献类型：
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作者：
BELL, JD;COX, IJ;URENJAK, J
用体外核磁共振P-31和H-1谱分析了人肝脏肿瘤的光谱变化和肿瘤对宿主肝脏可能的全身影响。肝脏肿瘤活检的H-1和P-31光谱显示，与组织学正常组织相比，磷酸乙醇胺、磷酸胆碱、牛磺酸、柠檬酸盐、丙氨酸、乳酸和甘氨酸显著升高，GPE（甘油磷酸乙醇胺）、GPC（甘油磷酸胆碱）、肌酸和苏氨酸显著降低。从荷瘤肝脏组织中获得的p -31核磁共振光谱显示，与非荷瘤患者（胰腺炎）的肝脏活检数据相比，磷酸乙醇胺和磷酸胆碱显著升高。这些结果表明，P-31-NMR可以检测宿主肝脏膜代谢的变化。
Spectral changes in human hepatic tumours and possible systemic effects of tumour on host liver were assessed by P-31 and H-1 in vitro NMR spectroscopy. The H-1 and P-31 spectra from liver tumour biopsies showed significant elevation in phosphoethanolamine, phosphocholine, taurine, citrate, alanine, lactate and glycine, and significant reduction in GPE (glycerophosphoethanol-amine), GPC (glycerophosphocholine), creatine and threonine compared to histologically normal tissue. P-31-NMR spectra obtained from histologically normal tissue within tumour-bearing livers showed significant elevation in phosphoethanolamine and phosphocholine compared to data from liver biopsies from nontumour-bearing patients (pancreatitis). These results suggest that alterations in membrane metabolism in host liver can be detected by P-31-NMR.