TRANSCRIPTIONAL REGULATION OF THE PROTON-TRANSLOCATING NADH DEHYDROGENASE GENES (NUOA-N) OF ESCHERICHIA-COLI BY ELECTRON-ACCEPTORS, ELECTRON-DONORS AND GENE REGULATORS
TRANSCRIPTIONAL REGULATION OF THE PROTON-TRANSLOCATING NADH DEHYDROGENASE GENES (NUOA-N) OF ESCHERICHIA-COLI BY ELECTRON-ACCEPTORS, ELECTRON-DONORS AND GENE REGULATORS
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DOI：
10.1111/j.1365-2958.1995.tb02416.x
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发表时间：
1995-05-01
影响因子：
3.6
通讯作者：
UNDEN, G
中科院分区：
文献类型：
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作者：
BONGAERTS, J;ZOSKE, S;UNDEN, G
nuoA-N基因位点编码的质子易位的NADH：醌氧化还原酶的启动子区域和转录调控进行了分析。nuo基因座上游的560 bp基因间区域之后是编码类似于LysR家族的基因调节子的基因（命名为LysR同源物A的IrhA）。IrhA的破坏并不影响生长（呼吸或非呼吸）或nuo的表达显着。通过电子受体、电子供体和转录调节因子ArcA、END、NarL和NarP以及IHF（整合宿主因子）对nuo的转录调节进行了研究，所述蛋白质和操纵子融合体含有高达碱基对-277（'nuo（277 ′））或高达碱基对-899（' nuo（899 ′）的启动子区域。nuo（277）-lacZ融合体的表达受到ArcA介导的厌氧抑制和NarL（+硝酸盐）介导的厌氧活化。FNR和ii-if在厌氧条件下作为弱阻遏物。nuo（899）-lacZ的表达在厌氧富马酸呼吸和有氧条件下由C-4二羧酸盐刺激。因此，nuo的表达在-277区域内的位点处通过ArcA、NarL、FNR和IHF由O-2和硝酸盐调节，并且在-277和-899之间的位点处由包括C-4二羧酸盐的其他因子调节。的转录刺激的生理作用的O-2和硝酸盐的建议。
The promoter region and transcriptional regulation of the nuoA-N gene locus encoding the proton-translocating NADH:quinone oxidoreductase was analysed. A 560 bp intergenic region upstream of the nuo locus was followed by a gene (designated IrhA for LysR homologue A) coding for a gene regulator similar to those of the LysR family. Disruption of IrhA did not affect growth (respiratory or non-respiratory) or expression of nuo significantly. Transcriptional regulation of nuo by electron accepters, electron donors and the transcriptional regulators ArcA, END, NarL and NarP, and by IHF (integration host factor) was studied with protein and operon fusions containing the promoter region up to base pair -277 ('nuo(277)') or up to base pair -899 ('nuo(899)'). The expression of the nuo(277)-lacZ fusions was subject to ArcA-mediated anaerobic repression and NarL(+ nitrate)-mediated anaerobic activation. FNR and ii-if acted as weak repressors under anaerobic conditions. Expression of nuo(899)-lacZ was stimulated during anaerobic fumarate respiration and aerobically by C-4 dicarboxylates. Therefore, expression of nuo is regulated by O-2 and nitrate via ArcA, NarL, FNR and IHF at sites within the -277 region, and by other factors including C-4 dicarboxylates at a site between -277 and -899. A physiological role for the transcriptional stimulation by O-2 and nitrate is suggested.