SEX-TYPING OF BLUE-CROWNED LAUGHINGTHRUSH (GARRLUAX COURTOISI) USING CHD1-BASED POLYMERASE CHAIN REACTION
SEX-TYPING OF BLUE-CROWNED LAUGHINGTHRUSH (GARRLUAX COURTOISI) USING CHD1-BASED POLYMERASE CHAIN REACTION
复制标题
使用基于 CHD1 的聚合酶链反应对蓝冠笑鸫 (Garrluax Courtoisi) 进行性别分型
DOI：
10.15666/aeer/1605_63416347
复制
发表时间：
2018
影响因子：
0.7
通讯作者：
YAN X
中科院分区：
文献类型：
--
作者：
HUANG J;MAO H;XU H;TANG J;OUYANG J;WU Z;LIU D;YAN X
蓝冠噪鹛（Garrluaxcourtoisi）是我国特有的濒危鸟类，性单态。这是很难确定的性别，通过观察其外部形态。为了解决这一问题，我们建立了一种基于CHD 1基因的PCR方法对墨头鱼进行性别分型。以南昌动物园饲养的6只蓝冠噪鹛为研究对象，采集血液样本，采用优化的酚/氯仿法从血液凝块中提取高质量的基因组DNA。我们设计了一个简并引物对扩增CHD 1基因的片段，导致700 bp的男性和600 bp的女性额外的PCR产物的扩增子。我们还对这六只鸟进行了广泛的行为观察，所获得的数据使我们能够推断这些鸟的性别。性别分型结果与行为推断结果基本一致。本研究为其他濒危鸟类的性别决定研究提供了理论依据，同时也为进一步研究墨头鸡的生殖机制、分类学分类和种群关系奠定了基础。
Blue-crowned laughingthrush (Garrluax courtoisi), an endemic species in China, is a critically endangered and sexually monomorphic bird. It is difficult to identify the gender of this species through the observation of its external morphology. To solve this problem, we herein established a CHD1-based PCR method for sex-typing of Garrluax courtoisi. We first collected blood samples from six Bluecrowned laughingthrush raised in Nanchang Zoo, and then extracted high-quality genomic DNA from blood clots using an optimized phenol/chloroform method. We designed a degenerate primer pair to amplify fragments of the CHD1 gene, resulting an amplicon of 700 bp for males and an extra PCR product of 600 bp for females. We also performed extensive behavioral observations of these six birds and the obtained data allowed us to deduce the sexes of these birds. The sex-typing results are generally consistent with the behavior-deduced results. This study sheds light on the sex determination of other endangered birds, and also paves a road to further test the reproduction mechanism, taxonomic classification and population relationships of Garrluax courtoisi.