Genetic dissection of epidermal growth factor receptor signaling during luteinizing hormone-induced oocyte maturation.
Genetic dissection of epidermal growth factor receptor signaling during luteinizing hormone-induced oocyte maturation.
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DOI：
10.1371/journal.pone.0021574
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Conti M
中科院分区：
文献类型：
--
作者：
Hsieh M;Thao K;Conti M
Recent evidence that luteinizing hormone (LH) stimulation of ovulatory follicles causes transactivation of the epidermal growth factor receptor (EGFR) has provided insights into the mechanisms of ovulation. However, the complete array of signals that promote oocyte reentry into the meiotic cell cycle in the follicle are still incompletely understood. To elucidate the signaling downstream of EGFR involved in oocyte maturation, we have investigated the LH responses in granulosa cells with targeted ablation of EGFR. Oocyte maturation and ovulation is disrupted when EGFR expression is progressively reduced. In granulosa cells from mice with either global or granulosa cell-specific disruption of EGFR signaling, LH-induced phosphorylation of MAPK3/1, p38MAPK, and connexin-43 is impaired. Although the LH-induced decrease in cGMP is EGFR-dependent in wild type follicles, LH still induces a decrease in cGMP in Egfrdelta/f Cyp19-Cre follicles. Thus compensatory mechanisms appear activated in the mutant. Spatial propagation of the LH signal in the follicle also is dependent on the EGF network, and likely is important for the control of signaling to the oocyte. Thus, multiple signals and redundant pathways contribute to regulating oocyte reentry into the cell cycle.
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影响因子：
15.9
作者：
Masciarelli, S;Horner, K;Manganiello, V
通讯作者：
Manganiello, V
影响因子：
4.6
作者：
Norris, Rachael P.;Freudzon, Marina;Mehlmann, Lisa M.;Cowan, Ann E.;Simon, Alexander M.;Paul, David L.;Lampe, Paul D.;Jaffe, Laurinda A.
通讯作者：
Jaffe, Laurinda A.
DOI：
10.1126/science.1171396
发表时间：
2009-05-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Fan HY;Liu Z;Shimada M;Sterneck E;Johnson PF;Hedrick SM;Richards JS
通讯作者：
Richards JS
影响因子：
56.9
作者：
Mehlmann, LM;Jones, TLZ;Jaffe, LA
通讯作者：
Jaffe, LA
影响因子：
2.7
作者：
Hinckley, M;Vaccari, S;Conti, M
通讯作者：
Conti, M