Molecular and cellular changes in skin and muscle during metamorphosis of Atlantic halibut (Hippoglossus hippoglossus) are accompanied by changes in deiodinases expression
Molecular and cellular changes in skin and muscle during metamorphosis of Atlantic halibut (Hippoglossus hippoglossus) are accompanied by changes in deiodinases expression
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DOI：
10.1007/s00441-012-1473-x
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发表时间：
2012-11-01
影响因子：
3.6
通讯作者：
Power, D. M.
中科院分区：
文献类型：
--
作者：
Campinho, M. A.;Galay-Burgos, M.;Power, D. M.
比目鱼变态是硬骨鱼类中最重要的发育事件。甲状腺激素（TH）、甲状腺素（T_4）和3，3 '-5'-三碘甲状腺原氨酸（T_3）是诱导和调节比目鱼变态的必要和充分因子。TH的大多数细胞和分子作用是通过T3与结合到启动子的甲状腺核受体结合来指导的，从而改变靶基因的表达。T4到T3的转化和T3的核可用性取决于3个硒代半胱氨酸脱碘酶家族的表达和活性，所述硒代半胱氨酸脱碘酶家族将T4活化成T3或降解T4和T3。我们研究了脱碘酶在大比目鱼皮肤和肌肉变性变化中的作用。我们发现，无论是在全身水平和在细胞水平在肌肉和皮肤的大西洋大比目鱼（Hippoglossus hippoglossus）在变态过程中，激活（D2）和失活（D3）脱碘酶的表达之间的协调是强烈相关的发展TH驱动的变化。D2和D3在皮肤和肌肉细胞中的表达模式表明，TH是维持这些组织中的幼虫变态发育和幼年细胞类型所必需的。在脱碘酶的表达和躯干皮肤和肌肉中发生的变态发育变化中没有对称性的断裂。这两个主要组织在幼虫和少年保持其对称性整个变态的研究结果表明，比目鱼变态过程中发生的不对称变化仅限于眼睛和头部区域。
Flatfish metamorphosis is the most dramatic post-natal developmental event in teleosts. Thyroid hormones (TH), thyroxine (T4) and 3,3'-5'-triiodothyronine (T3) are the necessary and sufficient factors that induce and regulate flatfish metamorphosis. Most of the cellular and molecular action of TH is directed through the binding of T3 to thyroid nuclear receptors bound to promoters with consequent changes in the expression of target genes. The conversion of T4 to T3 and nuclear availability of T3 depends on the expression and activity of a family of 3 selenocysteine deiodinases that activate T4 into T3 or degrade T4 and T3. We have investigated the role of deiodinases in skin and muscle metamorphic changes in halibut. We show that, both at the whole body level and at the cellular level in muscle and skin of the Atlantic halibut (Hippoglossus hippoglossus) during metamorphosis, the coordination between activating (D2) and deactivating (D3) deiodinases expression is strongly correlated with the developmental TH-driven changes. The expression pattern of D2 and D3 in cells of both skin and muscle indicate that TH are necessary for the maintenance of larval metamorphic development and juvenile cell types in these tissues. No break in symmetry occurs in the expression of deiodinases and in metamorphic developmental changes occurring both in trunk skin and muscle. The findings that two of the major tissues in both larvae and juveniles maintain their symmetry throughout metamorphosis suggest that the asymmetric changes occurring during flatfish metamorphosis are restricted to the eye and head region.