ATF-2 has intrinsic histone acetyltransferase activity which is modulated by phosphorylation
ATF-2 has intrinsic histone acetyltransferase activity which is modulated by phosphorylation
复制标题
DOI：
10.1038/35012097
复制
发表时间：
2000-05-11
期刊：
影响因子：
64.8
通讯作者：
Yokoyama, KK
中科院分区：
文献类型：
--
作者：
Kawasaki, H;Schiltz, L;Yokoyama, KK
转录因子携带功能结构域，这些功能结构域通常在物理上是不同的，用于序列特异性DNA结合、转录激活和调节功能。转录因子ATF-2是一种DNA结合蛋白，可与环状AMP反应元件（克雷斯）结合，与c-Jun形成同源二聚体或异源二聚体，并刺激CREs依赖性转录（1-3）。ATF-2是一种组蛋白乙酰转移酶（HAT），在体外特异性乙酰化组蛋白H2 B和H4。位于HAT结构域的基序A负责刺激CRE依赖的转录;此外，在响应紫外线照射时，ATF-2的磷酸化伴随着ATF-2和CRE依赖的转录的HAT活性增强。这些结果表明ATF-2的磷酸化控制其内在的HAT活性及其对CRE依赖性转录的作用。ATF-2可能代表了一类新的序列特异性因子，其能够通过直接作用于染色质组分来激活转录。
Transcription factors carry functional domains, which are often physically distinct, for sequence-specific DNA binding, transcriptional activation and regulatory functions. The transcription factor ATF-2 is a DNA-binding protein that binds to cyclic AMP-response elements (CREs), forms a homodimer or heterodimer with c-Jun, and stimulates CRE-dependent transcription(1-3). Here we report that ATF-2 is a histone acetyltransferase (HAT), which specifically acetylates histones H2B and H4 in vitro. Motif A, which is located in the HAT domain, is responsible for the stimulation of CRE-dependent transcription; moreover, in response to ultraviolet irradiation, phosphorylation of ATF-2 is accompanied by enhanced HAT activity of ATF-2 and CRE-dependent transcription. These results indicate that phosphorylation of ATF-2 controls its intrinsic HAT activity and its action on CRE-dependent transcription. ATF-2 may represent a new class of sequence-specific factors, which are able to activate transcription by direct effects on chromatin components.