Evaluation of the intestinal absorption of deoxynivalenol and nivalenol by an in vitro gastrointestinal model, and the binding efficacy of activated carbon and other adsorbent materials
Evaluation of the intestinal absorption of deoxynivalenol and nivalenol by an in vitro gastrointestinal model, and the binding efficacy of activated carbon and other adsorbent materials
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DOI：
10.1016/j.fct.2004.01.004
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发表时间：
2004-05-01
影响因子：
4.3
通讯作者：
Visconti, A
中科院分区：
文献类型：
--
作者：
Avantaggiato, G;Havenaar, R;Visconti, A
在体外筛选的14个吸附材料，包括一些商业产品用于解毒镰刀菌真菌毒素，进行了测试，在pH值范围为3-8的脱氧雪腐镰刀菌烯醇（DON）和雪腐镰刀菌烯醇（NIV）的结合能力。只有活性炭表现出有效的结合能力为35.1 μ mol和8.8 μ mol DON和NIV/g吸附剂，分别从吸附等温线计算。采用模拟健康猪胃肠道的动态实验室模型（TIM系统）评价DON和NIV的小肠吸收以及活性炭降低相关吸收的功效。在体外肠道吸收的DON和NIV分别为51%和21%，分别为170杯DON和230杯NIV摄入污染（加标）小麦。两种真菌毒素的大部分吸收发生在空肠腔室。活性炭的加入显著降低了肠道霉菌毒素的吸收。在2%的纳入水平的吸收相对于摄入量降低从51%至28%的DON和从21%至12%的NIV。活性炭的结合活性，这些烯类化合物是低于玉米赤霉烯酮，真菌毒素经常与他们共同发生在自然污染的谷物观察。(C)2004爱思唯尔有限公司保留所有权利。
In vitro screening of 14 adsorbent materials, including some commercial products used to detoxify Fusarium-mycotoxins, were tested in the pH range of 3-8 for deoxynivalenol (DON)- and nivalenol (NIV)-binding ability. Only activated carbon showed to be effective with binding capacities of 35.1 mumol and 8.8 mumol DON and NIV/g adsorbent, respectively, calculated from the adsorption isotherms. A dynamic laboratory model simulating the gastrointestinal (GI) tract of healthy pigs (TIM system) was used to evaluate the small-intestinal absorption of DON and NIV and the efficacy of activated carbon in reducing the relevant absorption. The in vitro intestinal absorptions of DON and NIV were 51% and 21%, respectively, as referred to 170 mug DON and 230 mug NIV ingested through contaminated (spiked) wheat. Most absorption occurred in the jejunal compartment for both mycotoxins. The inclusion of activated carbon produced a significant reduction in the intestinal mycotoxin absorption. At 2% inclusion level the absorption with respect to the intake was lowered from 51% to 28% for DON and from 21% to 12% for NIV. The binding activity of activated carbon for these trichothecenes was lower than that observed for zearalenone, a mycotoxin frequently co-occurring with them in naturally contaminated cereals. (C) 2004 Elsevier Ltd. All rights reserved.