RNA-binding protein MAC5A interacts with the 26S proteasome to regulate DNA damage response in Arabidopsis
RNA-binding protein MAC5A interacts with the 26S proteasome to regulate DNA damage response in Arabidopsis
复制标题
RNA结合蛋白MAC5A与26S蛋白酶体相互作用调节拟南芥DNA损伤反应
DOI：
10.1093/plphys/kiac510
复制
发表时间：
2022-11-04
期刊：
影响因子：
7.4
通讯作者：
Li, Shengjun
中科院分区：
文献类型：
--
作者：
Meng, Xiangxiang;Wang, Quanhui;Li, Shengjun
真核生物的DNA损伤反应(DDR)对于在具有挑战性的环境中保持基因组的完整性是必不可少的。DDR的调节机制已经在酵母和人类中得到了很好的建立。然而，越来越多的证据支持这样一种观点，即植物似乎使用了在很大程度上仍不为人所知的不同信号通路。在这里，我们报道了SNC1，4相关复合亚单位5A的修饰物(MAC5A)在拟南芥DDR中的作用。在mac5a突变体中，MAC5A的缺失导致了对DNA损伤诱导剂甲烷磺酸甲酯(MMS)的超敏表型。与这一观察结果一致，MAC5A可以调节DDR基因的选择性剪接，以维持对遗传毒性应激的适当反应。有趣的是，MAC5A与26S蛋白酶体(26SP)相互作用，是其蛋白酶体活性所必需的。MAC核心亚单位也参与了MMS诱导的DDR。此外，我们发现MAC5A、MAC核心亚单位和26SP可能协同作用，通过DDR介导高硼诱导的生长抑制。综上所述，我们的发现揭示了MAC在MMS诱导的DDR中在植物生长和胁迫适应中的关键作用。MOS4相关的复合亚单位5A与26S蛋白酶体相互作用，并参与拟南芥对甲烷磺酸甲酯和高硼胁迫诱导的DNA损伤反应。
DNA damage response (DDR) in eukaryotes is essential for the maintenance of genome integrity in challenging environments. The regulatory mechanisms of DDR have been well-established in yeast and humans. However, increasing evidence supports the idea that plants seem to employ different signaling pathways that remain largely unknown. Here, we report the role of MODIFIER OF SNC1, 4-ASSOCIATED COMPLEX SUBUNIT 5A (MAC5A) in DDR in Arabidopsis (Arabidopsis thaliana). Lack of MAC5A in mac5a mutants causes hypersensitive phenotypes to methyl methanesulfonate (MMS), a DNA damage inducer. Consistent with this observation, MAC5A can regulate alternative splicing of DDR genes to maintain the proper response to genotoxic stress. Interestingly, MAC5A interacts with the 26S proteasome (26SP) and is required for its proteasome activity. MAC core subunits are also involved in MMS-induced DDR. Moreover, we find that MAC5A, the MAC core subunits, and 26SP may act collaboratively to mediate high-boron-induced growth repression through DDR. Collectively, our findings uncover the crucial role of MAC in MMS-induced DDR in orchestrating growth and stress adaptation in plants.MOS4-ASSOCIATED COMPLEX SUBUNIT 5A interacts with the 26S proteasome and is involved in DNA damage response induced by methyl methanesulfonate and high-boron stress in Arabidopsis.