Laccase2 is required for cuticular pigmentation in stinkbugs
Laccase2 is required for cuticular pigmentation in stinkbugs
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DOI：
10.1016/j.ibmb.2010.12.003
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发表时间：
2011-03-01
影响因子：
3.8
通讯作者：
Fukatsu, Takema
中科院分区：
文献类型：
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作者：
Futahashi, Ryo;Tanaka, Kohjiro;Fukatsu, Takema
在昆虫角质层成熟过程中，蛋白质-蛋白质和蛋白质-甲壳素在二酚氧化酶的作用下形成交联键。昆虫角质层中存在两种类型的二酚氧化酶，即漆酶和酪氨酸酶。在鞘翅目和膜翅目昆虫中，漆酶2基因编码一种主要负责角质层着色和硬化的酶，而漆酶基因在半代谢昆虫中的生物学作用尚不清楚。在这里，我们从三种半翅目臭虫中鉴定出漆酶2基因，它们是：Riptortus Hadstris(Alydidae)，Nysius plebeius(Lygaeidae)和Megacopta marktatisima(Plataspidae)。在胡枝子中，漆酶2基因在蜕皮前的表皮组织中高度表达。当基因表达被RNA干扰技术抑制时，蜕皮后的皮肤色素沉着被阻止，这取决于针对漆酶2基因的双链RNA注射的剂量。普氏原虫和马尾藻也得到了类似的结果。在所有种类的臭虫中，注射20 ng双链RNA足以阻止角质层成熟。这些结果表明，漆酶2基因在不同的臭虫家族中普遍需要皮肤色素沉着，这突出了它在不同昆虫类群中保守的生物学功能。(C)2010爱思唯尔有限公司。保留所有权利。
During the maturation of insect cuticle, protein-protein and protein-chitin crosslinkages are formed by the action of diphenoloxidases. Two types of diphenoloxidases, laccases and tyrosinases, are present in the insect cuticle. In coleopteran and hymenopteran insects, laccase2 gene has been identified as encoding an enzyme principally responsible for cuticular pigmentation and hardening, whereas biological roles of laccase genes in hemimetabolous insects remain to be established. Here we identified laccase2 genes from three hemipteran stinkbugs, Riptortus pedestris (Alydidae), Nysius plebeius (Lygaeidae) and Megacopta punctatissima (Plataspidae). In R. pedestris, laccase2 gene was highly expressed in epidermal tissues prior to molting. When the gene expression was suppressed by an RNA interference technique, cuticular pigmentation after molting were blocked depending on the dose of injected double-stranded RNA targeting the laccase2 gene. Similar results were obtained for N. plebeius and M. punctatissima. In all the stinkbug species, injecting 20 ng of double-stranded RNA was sufficient to prevent the cuticular maturation. These results indicate that laccase2 gene is generally required for cuticular pigmentation in different stinkbug families, highlighting its conserved biological function across diverse insect taxa. (C) 2010 Elsevier Ltd. All rights reserved.