A pectin from fruits of Lycium barbarum L. decreases β-amyloid peptide production through modulating APP processing.
A pectin from fruits of Lycium barbarum L. decreases β-amyloid peptide production through modulating APP processing.
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DOI：
10.1016/j.carbpol.2018.08.050
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发表时间：
2018-12
影响因子：
11.2
通讯作者：
Lishuang Zhou;Wenfeng Liao;Hui Zeng;Yanli Yao;Xia Chen;K. Ding
中科院分区：
文献类型：
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作者：
Lishuang Zhou;Wenfeng Liao;Hui Zeng;Yanli Yao;Xia Chen;K. Ding
这里，从枸杞果实中纯化出分子量为99.8kDa的果胶LBP1C-2。其结构被阐明为交替 1, 2-连接 α-Rhap 和 1, 4-连接 α-GalpA 的主链，末端分支为 (T) -, 1, 3-, 1, 6- 和 1, 3, 6-连接 β-Galp, T-, 1, 5- 和 1, 3, 5-连接 α-Araf 和 T-连接 β-Rhap 在 C-4 处取代1、2、4 连接的 α-Rhap。基于细胞的实验表明，LBP1C-2 以剂量依赖性方式抑制 Aβ42 的产生，且没有细胞毒性。进一步研究表明，LBP1C-2 减弱了 β 位点 APP 裂解酶 1 (BACE1) 的表达，而 LBP1C-2 则上调了 ADAM10 的表达。此外，LBP1C-2 促进胰岛素降解酶（IDE）的表达。这些结果表明，LBP1C-2 可能通过介导 BACE1 和 ADAM10 以及 IDE 表达来减少 Aβ42 的产生，从而成为抗阿尔茨海默病治疗的主要化合物。
Here, a pectin LBP1C-2 with the molecular weight of 99.8 kDa was purified from fruits ofLycium barbarumL. Its structure was elucidated as a backbone of alternate 1, 2-linked α-Rhapand 1, 4-linked α-GalpA, with branches of terminal (T) -, 1, 3-, 1, 6- and 1, 3, 6-linked β-Galp, T-, 1, 5- and 1, 3, 5-linked α-Arafand T-linked β-Rhapsubstituted at C-4 of 1, 2, 4-linked α-Rhap. The cell-based experiments indicated that LBP1C-2 suppressed Aβ42production in a dose-dependent manner with no cytotoxicity. Further study showed that expression of β-site APP cleaving enzyme 1 (BACE1) was attenuated by LBP1C-2, while expression of ADAM10 was up-regulated by LBP1C-2. Moreover, LBP1C-2 promoted the expression of insulin-degradation enzyme (IDE). These results suggested that LBP1C-2 might be a leading compound for anti-Alzheimer’s disease therapy by decreasing Aβ42production through mediating BACE1 and ADAM10 as well as IDE expression.