Testis lesions and cellular and humoral immune responses induced in rats by immunization with laminin.
Testis lesions and cellular and humoral immune responses induced in rats by immunization with laminin.
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层粘连蛋白免疫诱导大鼠睾丸损伤以及细胞和体液免疫反应。
DOI：
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发表时间：
1987
影响因子：
3.6
通讯作者：
B. Denduchis
中科院分区：
文献类型：
--
作者：
L. Lustig;G. Doncel;E. Berensztein;B. Denduchis
用分离自小鼠Engelbreth-Holm-Swarm（EHS）肉瘤的层粘连蛋白免疫的大鼠中，66%在睾丸中出现中度病变，其特征为多个曲细精小管病灶，伴有不同程度的生发上皮脱落或萎缩，与正常组织学区域混合。附睾间质单核细胞浸润。电镜下可见曲细精管基底膜的病理变化，如突起分裂、增厚。支持细胞胞质内可见扩张的滑面内质网和大空泡。通过电子显微镜与免疫过氧化物酶技术，染色在体内结合的大鼠IgG检测到沿着曲细精管壁作为一个明亮的线性免疫荧光和作为一个致密的反应产物在基膜上。ELISA法检测到高滴度的循环抗层粘连蛋白抗体在所有的大鼠免疫层粘连蛋白。如皮肤试验所示，在这些大鼠中观察到对层粘连蛋白的迟发型超敏反应。
Sixty-six percent of rats immunized with laminin isolated from a mouse Engelbreth-Holm-Swarm (EHS) sarcoma developed moderate lesions in the testis characterized by multiple foci of seminiferous tubules with different degrees of sloughing of the germinal epithelium or atrophy intermingled with normal histological areas. Interstitial mononuclear cell infiltrates were seen in the epididymis. By electron microscopy, pathological changes in the basement membranes of the seminiferous tubules, such as splitting and focal thickenings of knob-like projections toward the epithelium, were observed. Moreover, Sertoli cell cytoplasm showed dilated smooth endoplasmic reticulum and large vacuoles. By electron microscopy with the immunoperoxidase technique, staining for in vivo-bound rat IgG was detected along the walls of the seminiferous tubules as a bright linear immunofluorescence and as a dense reaction product on the basal lamina. High titers of circulating antilaminin antibodies were detected by ELISA in all the rats immunized with laminin. As revealed by the skin test, a delayed type hypersensitivity reaction to laminin was observed in these rats.
影响因子：
13.2
作者：
Ohlsson, Sophie;Herlitz, Hans;Segelmark, Marten
通讯作者：
Segelmark, Marten